By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Mosquito-Borne Diseases. According to news reporting originating from Notre Dame, Indiana, by NewsRx correspondents, research stated, "Recent epidemics of dengue viruses (DENV) coupled with new outbreaks on the horizon have renewed the demand for novel detection methods that have the ability to identify this viral pathogen prior to the manifestation of symptoms. The ability to detect DENV in a timely manner is essential for rapid recovery from disease symptoms."
Our news editors obtained a quote from the research from the University of Notre Dame, "A modified lab-derived 10-23 DNAzyme tethered to gold nanoparticles provides a powerful tool for the detection of viruses, such as DENV. We examined the effectiveness of coupling DNAzyme (DDZ) activation to the salt-induced aggregation of gold nanoparticles (AuNP) to detect dengue virus (DENV) progeny in mosquito cells. A DNAzyme was designed to recognize the 5' cyclization sequence (5' CS) that is conserved among all DENV, and conjugated to AuNPs. DDZ-AuNP has demonstrated the ability to detect the genomic RNA of our model dengue strain, DENV-2 NGC, isolated from infected Aedes albopictus C6/36 cells. These targeting events lead to the rapid aggregation of AuNPs, resulting in a red to clear color transition of the reaction mixes, and thus positive detection of the DENV RNA genome. The inclusion of SDS in the reaction mixture permitted the detection of DENV directly from cell culture supernatants without additional sample processing. Specificity assays demonstrated detection is DENV-specific, while sensitivity assays confirm detection at levels of 1 x 10(1) TCID50 units. These results demonstrate DDZ-AuNP effectively detects DENV genomes in a sequence specific manner and at concentrations that are practical for field use. We have developed an effective detection assay using DNAzyme catalysis coupled with AuNP aggregation for the detection of DENV genomes in a sequence specific manner."
According to the news editors, the research concluded: "Full development of our novel DDZ-AuNP detection method will provide a practical, rapid, and low cost alternative for the detection of DENV in mosquito cells and tissues, and possibly infected patient serum, in a matter of minutes with little to no specialized training required."
For more information on this research see: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches. Virology Journal, 2013;10():1-15. Virology Journal can be contacted at: Biomed Central Ltd, 236 Grays Inn Rd, Floor 6, London WC1X 8HL, England. (BioMed Central - www.biomedcentral.com/; Virology Journal - www.virologyj.com)
The news editors report that additional information may be obtained by contacting J.R. Carter, University of Notre Dame, Dept. of Biol Sci, Eck Inst Global Hlth, Notre Dame, IN 46556, United States. Additional authors for this research include V. Balaraman, C.A. Kucharski, T.S. Fraser and M.J. Fraser (see also Mosquito-Borne Diseases).
Keywords for this news article include: Indiana, Virology, Notre Dame, RNA Viruses, Dengue Fever, United States, Nanotechnology, Gold Nanoparticles, Arbovirus Infections, Emerging Technologies, Flavivirus Infections, Flaviviridae Infections, Mosquito-Borne Diseases, North and Central America
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC