By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on Stem Cell Research. According to news originating from Winnipeg, Canada, by NewsRx correspondents, research stated, "Central nervous system development is controlled by highly conserved homeoprotein transcription factors including HOX and TALE (Three Amino acid Loop Extension). TALE proteins are primarily known as HOX-cofactors and play key roles in cell proliferation, differentiation and organogenesis."
Our news journalists obtained a quote from the research from the University of Manitoba, "MEIS1 is a TALE member with established expression in the developing central nervous system. MEIS1 is essential for embryonic development and Meis1 knockout mice dies at embryonic day (E) 14.5. However, Meis1/MEIS1 expression in the devolving forebrain, at this critical time-point has not been studied. Here, for the first time we characterize the region-specific expression of MEIS1 in E14.5 mouse forebrain, filling the gap of MEIS1 expression profile between E12.5 and E16.5. Previously, we reported MEIS1 transcriptional regulatory role in neuronal differentiation and established forebrain-derived neural stem cells (NSC) for gene therapy application of neuronal genes. Here, we show the dynamic expression of Meis1/MEIS1 during the differentiation of forebrain-derived NSC toward a glial lineage. Our results show that Meis1/MEIS1 expression is induced during NSC differentiation and is expressed in both differentiated neurons and astrocytes. Confirming these results, we detected MEIS1 expression in primary cultures of in vivo differentiated cortical neurons and astrocytes. We further demonstrate Meis1/MEIS1 expression relative to other TALE family members in the forebrain-derived NSC in the absence of Hox genes."
According to the news editors, the research concluded: "Our data provide evidence that forebrain-derived NSC can be used as an accessible in vitro model to study the expression and function of TALE proteins, supporting their potential role in modulating NSC self-renewal and differentiation."
For more information on this research see: Dynamic expression of MEIS1 homeoprotein in E14.5 forebrain and differentiated forebrain-derived neural stem cells. Annals of Anatomy-Anatomischer Anzeiger, 2013;195(5):431-440. Annals of Anatomy-Anatomischer Anzeiger can be contacted at: Elsevier Gmbh, Urban & Fischer Verlag, Office Jena, P O Box 100537, 07705 Jena, Germany (see also Stem Cell Research).
The news correspondents report that additional information may be obtained from B.A. Barber, University of Manitoba, Regenerat Med Program, Fac Med, Dept. of Biochem & Med Genet, Winnipeg, MB R3E 0J9, Canada. Additional authors for this research include V.R.B. Liyanage, R.M. Zachariah, C.O. Olson, M.A.G. Bailey and M. Rastegar.
Keywords for this news article include: Biotechnology, Canada, Winnipeg, Manitoba, Gene Therapy, Bioengineering, Stem Cell Research, North and Central America
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