Our news journalists obtained a quote from the research from Sanofi Pasteur, "The cell line AV529-19 is a Vero-based cell line specifically engineered to express the HSV-1 UL5 and UL29 open reading frames. AV529-19 is used to support the replication of a defective HSV-2 viral candidate vaccine called HSV529. To assess the genetic stability of the UL5 and UL29 transgenes in AV529-19 cells, a digital PCR-based approach was developed. During characterization of the test method, the specificity, accuracy, and intermediate precision of the assay was investigated based on regulatory guidelines. The developed assay was used to monitor the stability of the transgenes in the manufactured AV529-19 cell lines by comparison of transgene copy numbers in the master cell bank (MCB) with their copy numbers in the extended cell bank (ECB)."
According to the news editors, the research concluded: "Results showed that the UL29 and UL5 transgenes are stable in that there are one and three copies of the UL29 and UL5 genes, respectively, per cell in both the AV529-19 MCB and ECB."
For more information on this research see: Determination of HSV-1 UL5 and UL29 gene copy numbers in an HSV complementing Vero cell line.
Our news journalists report that additional information may be obtained by contacting A. Azizi, Sanofi Pasteur,
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Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC
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