By a News Reporter-Staff News Editor at Science Letter -- Current study results on DNA Research have been published. According to news reporting originating in Lexington, Kentucky, by NewsRx journalists, research stated, "Over the past decade, nanopores have rapidly emerged as stochastic biosensors. This protocol describes the cloning, expression and purification of the channel of the bacteriophage phi29 DNA-packaging nanomotor and its subsequent incorporation into lipid membranes for single-pore sensing of double-stranded DNA (dsDNA) and chemicals."
The news reporters obtained a quote from the research from the University of Kentucky, "The membrane-embedded phi29 nanochannel remains functional and structurally intact under a range of conditions. When ions and macromolecules translocate through this nanochannel, reliable fingerprint changes in conductance are observed. Compared with other well-studied biological pores, the phi29 nanochannel has a larger cross-sectional area, which enables the translocation of dsDNA. Furthermore, specific amino acids can be introduced by site-directed mutagenesis within the large cavity of the channel to conjugate receptors that are able to bind specific ligands or analytes for desired applications. The lipid membrane-embedded nanochannel system has immense potential nanotechnological and biomedical applications in bioreactors, environmental sensing, drug monitoring, controlled drug delivery, early disease diagnosis and high-throughput DNA sequencing."
According to the news reporters, the research concluded: "The total time required for completing one round of this protocol is around 1 month."
For more information on this research see: Incorporation of a viral DNA-packaging motor channel in lipid bilayers for real-time, single-molecule sensing of chemicals and double-stranded DNA. Nature Protocols, 2013;8(2):373-92. Nature Protocols can be contacted at: Nature Publishing Group, 345 Park Avenue South, New York, NY 10010-1707, USA. (Nature Publishing Group - www.nature.com/; Nature Protocols - www.nature.com/nprot/)
Our news correspondents report that additional information may be obtained by contacting F. Haque, College of Pharmacy, University of Kentucky, Lexington, Kentucky, United States. Additional authors for this research include J. Geng, C. Montemagno and P. Guo (see also DNA Research).
The publisher of the journal Nature Protocols can be contacted at: Nature Publishing Group, 345 Park Avenue South, New York, NY 10010-1707, USA.
Keywords for this news article include: Kentucky, Lexington, Chemicals, Chemistry, DNA Research, United States, North and Central America.
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