By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Nanoparticles is now available. According to news reporting originating in College Station, Texas, by NewsRx journalists, research stated, "The Fe content of Jurkat cells grown on transferrin-bound iron (TBI) and Fe(III) citrate (FC) was characterized using Mossbauer, electron paramagnetic resonance, and UV-vis spectroscopies, as well as electron and inductively coupled plasma mass spectrometry. Isolated mitochondria were similarly characterized."
The news reporters obtained a quote from the research from Texas A&M University, "Fe-limited cells contained ~100 ?M essential Fe, mainly as mitochondrial Fe and nonmitochondrial non-heme high-spin Fe(II). Cells replete with Fe also contained ferritin-bound Fe and Fe(III) oxyhydroxide nanoparticles. Only 400 ± 100 Fe ions were loaded per ferritin complex, regardless of the growth medium Fe concentration. Ferritin regulation thus appears to be more complex than is commonly assumed. The magnetic and structural properties of Jurkat nanoparticles differed from those of yeast mitochondria. They were smaller and may be located in the cytosol. The extent of nanoparticle formation scaled nonlinearly with the concentration of Fe in the medium. Nanoparticle formation was not strongly correlated with reactive oxygen species (ROS) damage. Cells could utilize nanoparticle Fe, converting such aggregates into essential Fe forms. Cells grown on galactose rather than glucose respired faster, grew slower, exhibited more ROS damage, and generally contained more nanoparticles. Cells grown with TBI rather than FC contained less Fe overall, more ferritin, and fewer nanoparticles. Cells in which the level of transferrin receptor expression was increased contained more ferritin Fe. Frataxin-deficient cells contained more nanoparticles than comparable wild-type cells. Data were analyzed by a chemically based mathematical model. Although simple, it captured essential features of Fe import, trafficking, and regulation. TBI import was highly regulated, but FC import was not."
According to the news reporters, the research concluded: "Nanoparticle formation was not regulated, but the rate was third-order in cytosolic Fe."
For more information on this research see: Mossbauer study and modeling of iron import and trafficking in human jurkat cells. Biochemistry, 2013;52(45):7926-42. Biochemistry can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (American Chemical Society - www.acs.org; Biochemistry - www.pubs.acs.org/journal/bichaw)
Our news correspondents report that additional information may be obtained by contacting N.D. Jhurry, Dept. of Biochemistry and Biophysics, Texas A&M University , College Station, Texas 77843-2128, United States. Additional authors for this research include M. Chakrabarti, S.P. McCormick, V.M. Gohil and P.A Lindahl (see also Nanoparticles).
The publisher of the journal Biochemistry can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Texas, United States, Nanotechnology, College Station, Emerging Technologies, North and Central America.
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