By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Enzymes and Coenzymes. According to news originating from Changsha, People's Republic of China, by NewsRx correspondents, research stated, "A new sensitive fluorometric assay method for acetylcholinesterase (AChE) and its inhibitor was developed using a fluorescent dye, nile red (NR). Due to the fluorescence resonance energy transfer between the NR and the gold nanoparticle (AuNPs), the fluorescence was quenched."
Our news journalists obtained a quote from the research from Hunan University, "AChE can break down acetylthiocholine to produce a thiol-bearing compound, thiocholine. In the presence of thiocholine, the nile red is replaced from the AuNPs surfaces and simultaneously transformed to a derivative of nile red. The fluorescence intensity of the derivative is much stronger than that of the native nile red with the same concentration and its maximum emission wavelength has a blue shift so that the sensor achieves a good signal-to-background ratio. In addition, when organophosphate pesticide (OPs) exists, the activity of AChE can be inhibited, the generation of thiocholine will be prevented and no fluorescence enhancement occurs."
According to the news editors, the research concluded: "The results show that the method is sensitive to AChE and paraoxon with the detection limits of 0.2 mU/mL and 0.05 ng/mL, respectively."
For more information on this research see: Highly Sensitive Fluorometric Assay Method for Acetylcholinesterase Inhibitor Based on Nile Red-Adsorbed Gold Nanoparticles. Chinese Journal of Chemistry, 2013;31(8):1072-1078. Chinese Journal of Chemistry can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell - www.wiley.com/; Chinese Journal of Chemistry - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1614-7065)
The news correspondents report that additional information may be obtained from W.T. Han, Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, People's Republic of China. Additional authors for this research include S.Z. Liao, C.H. Zhang, H.Z. Ding, Z.Y. Wu, G.L. Shen and R.Q. Yu (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Amines, Changsha, Hydrolases, Thiocholine, Nanotechnology, Organic Chemicals, Gold Nanoparticles, Acetylcholinesterase, Sulfhydryl Compounds, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China, Quaternary Ammonium Compounds
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