By a News Reporter-Staff News Editor at Angiogenesis Weekly -- Current study results on Biomedicine and Biomedical Engineering have been published. According to news reporting originating in Boston, Massachusetts, by NewsRx journalists, research stated, "Development of a pre-vascularized tissue-engineered construct with intrinsic vascular system for cell growth and tissue formation still faces many difficulties due to the complexity of the vascular network of natural bone tissue. The present study was to design and form a new vascularized tissue-engineered construct using pre-differentiated rADSCs, arteriovenous vascular bundle and porous nHA-PA 66 scaffold."
The news reporters obtained a quote from the research from Massachusetts General Hospital, "rADSCs were pre-differentiated to endothelial cells (rADSCs-Endo) and then incorporated in nHA-PA 66 scaffolds in vitro. Subsequently, in vivo experiments were carried out according to the following groups: Group A (rADSCs-Endo/nHA-PA 66 scaffold with arteriovenous vascular bundle), Group B (rADSCs/nHA-PA 66 scaffold with arteriovenous vascular bundle); Group C (nHA-PA66 scaffold with arteriovenous vascular bundle), Group D (nHA-PA 66 scaffold only). The vessel density and vessel diameter were measured based on histological and immunohistochemical evaluation, furthermore, the VEGF-C, FGF-2 and BMP-2 protein expressions were also evaluated by western blot analysis. The results of in vivo experiments showed that the vessel density and vessel diameter in group A were significantly higher than the other three groups. Between Group B and C, no statistical difference was observed at each time point. In accordance with the results, there were dramatically higher expressions of VEGF-C and FGF-2 protein in Group A than that of Group B, C and D at 2 or 4 weeks. Statistical differences were not observed in VEGF-C and FGF-2 expression between Group B and C. BMP-2 was not expressed in any group at each time point. Compared with muscular wrapping method, arteriovenous vascular bundle implantation could promote vascularization of the scaffold; and the angiogenesis of the scaffold was significantly accelerated when pre-differentiated rADSCs (endothelial differentiation) were added."
According to the news reporters, the research concluded: "These positive results implicate the combination of pre-differentiated rADSCs (endothelial differentiation) and arteriovenous vascular bundle may achieve rapidly angiogenesis of biomaterial scaffold."
For more information on this research see: Development of a new pre-vascularized tissue-engineered construct using pre-differentiated rADSCs, arteriovenous vascular bundle and porous nano-hydroxyapatide-polyamide 66 scaffold. BMC Musculoskeletal Disorders, 2013;14():1-14. BMC Musculoskeletal Disorders can be contacted at: Biomed Central Ltd, 236 Grays Inn Rd, Floor 6, London WC1X 8HL, England. (BioMed Central - www.biomedcentral.com/; BMC Musculoskeletal Disorders - www.biomedcentral.com/bmcmusculoskeletdisord/)
Our news correspondents report that additional information may be obtained by contacting P. Yang, Massachusetts General Hospital, Sarcoma Biol Lab, Center Sarcoma & Connect Tissue Oncol, Boston, MA 02114, United States. Additional authors for this research include X. Huang, J. Shen, C.S. Wang, X.Q. Dang, H. Mankin, Z.F. Duan and K.Z. Wang (see also Biomedicine and Biomedical Engineering).
Keywords for this news article include: VEGF, Tissue Engineering, Biomedicine and Biomedical Engineering, Boston, Massachusetts, United States, Bioengineering, Protein Kinases, Membrane Proteins, Angiogenic Proteins, Phosphotransferases, Growth Factor Receptors, North and Central America, Receptor Protein-Tyrosine Kinases, Vascular Endothelial Growth Factors
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