By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Biotechnology have been presented. According to news reporting out of Shenzhen, People's Republic of China, by NewsRx editors, research stated, "To develop an effective and easy-to-administer vaccine against vibriosis of fish, the chitosan nanoparticles-loaded DNA vaccine against Vibrio parahaemolyticus was studied. A DNA vaccine was constructed using the outer membrane protein K (ompK) gene of V. parahaemolyticus strain (OS4) and pEGFP-N2 , a eukaryotic expression vector, and the construct was named pEGFP-N2 -OMPK (pDNA)."
Our news journalists obtained a quote from the research from Shenzhen University, "The pDNA was encapsulated in chitosan particles (chitosan/pDNA). The effective diameter, mean diameter and polydispersity of the particles were 284.4 nm, 218.9 nm and 0.160, respectively. Scanning electron microscopy showed that the particles are dispersed as individual nanoparticles with spherical shape of around 200 nm and are homogeneously distributed. Encapsulation efficiency and loading percentage of nanoparticles were 91.5% and 2.08%, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) showed that RNA-containing information of the ompK gene existed in mid-intestine, liver, kidney and muscle 3 weeks after oral administration in black seabream Acanthopagrus schlegelii Bleeker. Expression of the reporter gene, green fluorescent protein (GFP), was observed in the above-mentioned tissues by fluorescence microscopy. Expression of the ompK gene within 3 weeks evoked an immune response. Black seabream was protected from V. parahaemolyticus (OS4), with 72.3% relative percentage survival (RPS) 3 weeks post-vaccination with chitosan/pDNA. The direct agglutination test indicated that oral administration with chitosan/pDNA induced an antibody immune response in fish against V. parahaemolyticus (OS4)."
According to the news editors, the research concluded: "Data obtained, here and in other related studies, suggest that chitosan nanoparticles are promising carriers for an oral pDNA vaccine."
For more information on this research see: Potential use of chitosan nanoparticles for oral delivery of DNA vaccine in black seabream Acanthopagrus schlegelii Bleeker to protect from Vibrio parahaemolyticus. Journal of Fish Diseases, 2013;36(12):987-95. (Wiley-Blackwell - www.wiley.com/; Journal of Fish Diseases - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2761)
Our news journalists report that additional information may be obtained by contacting L. Li, College of Life Sciences, Shenzhen Key Laboratory of Marine Biological Resources and Ecological Environment, Shenzhen University, Shenzhen, Guangdong Province, People's Taiwan. Additional authors for this research include S.L. Lin, L. Deng and Z.G Liu (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Shenzhen, DNA Research, DNA Vaccines, Nanoparticle, Vibrionaceae, Nanotechnology, Synthetic Vaccines, Gammaproteobacteria, Emerging Technologies, Gram Negative Bacteria, Vibrio parahaemolyticus, People's Republic of China, Gram Negative Facultatively Anaerobic Rods.
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