By a News Reporter-Staff News Editor at Science Letter -- Research findings on Science are discussed in a new report. According to news originating from Durham, North Carolina, by NewsRx correspondents, research stated, "As the designs of polymer systems used to deliver nucleic acids continue to evolve, it is becoming increasingly apparent that the basic bulk manufacturing techniques of the past will be insufficient to produce polymer-nucleic acid nanocomplexes that possess the uniformity, stability, and potency required for their successful clinical translation and widespread commercialization. Traditional bulk-prepared products are often physicochemically heterogeneous and may vary significantly from one batch to the next."
Our news journalists obtained a quote from the research from Duke University, "Here we show that preparation of bioreducible nanocomplexes with an emulsion-based droplet microfluidic system produces significantly improved nanoparticles that are up to fifty percent smaller, more uniform, and are less prone to aggregation. The intracellular integrity of nanocomplexes prepared with this microfluidic method is significantly prolonged, as detected using a high-throughput flow cytometric quantum dot Forster resonance energy transfer nanosensor system. These physical attributes conspire to consistently enhance the delivery of both plasmid DNA and messenger RNA payloads in stem cells, primary cells, and human cell lines."
According to the news editors, the research concluded: "Innovation in processing is necessary to move the field toward the broader clinical implementation of safe and effective nonviral nucleic acid therapeutics, and preparation with droplet microfluidics represents a step forward in addressing the critical barrier of robust and reproducible nanocomplex production."
For more information on this research see: Microfluidic preparation of polymer-nucleic acid nanocomplexes improves nonviral gene transfer. Scientific Reports, 2013;3():3155. (Nature Publishing Group - www.nature.com/; Scientific Reports - www.nature.com/srep/)
The news correspondents report that additional information may be obtained from C.L. Grigsby, Dept. of Biomedical Engineering, Duke University, 136 Hudson Hall, Box 90281, Durham, NC 27708, United States. Additional authors for this research include Y.P. Ho, C. Lin, J.F. Engbersen and K.W Leong (see also Science).
Keywords for this news article include: Durham, Science, Therapeutics, United States, North Carolina, North and Central America.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC