By a News Reporter-Staff News Editor at Science Letter -- Data detailed on Immunology have been presented. According to news reporting originating from Karlsruhe, Germany, by NewsRx correspondents, research stated, "Protein drugs continue to grow both in medicinal importance as in scale of their production. This furthers the interest in separation technologies that have the potential to replace chromatographic steps in a protein purification process."
Our news editors obtained a quote from the research from the Karlsruhe Institute of Technology, "Two such unit operations that are employed in large scale in the chemical industry are extraction and precipitation. Their usefulness for the purification of proteins has been demonstrated, but the integration of such unit operations in a way that generate an output stream of high protein concentration and low process related impurities was missing. In this work, we employ centrifugal partitioning chromatography ('CPC') in combination with precipitation of the protein of interest to purify a cell culture supernatant of a monoclonal antibody producing cell line. Centrifugal partitioning chromatography was used as means of multi-step extraction using aqueous two-phase systems and was able to remove up to 88.2% of host cell protein ('HCP'). The following PEG driven precipitation and resolubilization of the protein of interest was use to condition the CPC output stream to suit subsequent chromatographic steps, to increase mAb concentration, remove the phase forming polymer, further improve HCP clearance, and integrate a low pH hold step for viral clearance. The entire process reduced HCP content by 99.4% while recovering 93% of the protein of interest."
According to the news editors, the research concluded: "High throughput screening techniques were extensively employed during the development of the process."
For more information on this research see: Alternative separation steps for monoclonal antibody purification: Combination of centrifugal partitioning chromatography and precipitation. Journal of Chromatography A, 2013;1319():118-126. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)
The news editors report that additional information may be obtained by contacting S.A. Oelmeier, Karlsruhe Inst Technol, Inst Proc Engn Life Sci, Sect Biomol Separat Engn 4, D-76021 Karlsruhe, Germany. Additional authors for this research include C. Ladd-Effio and J. Hubbuch (see also Immunology).
Keywords for this news article include: Antibodies, Europe, Germany, Therapy, Karlsruhe, Immunology, Blood Proteins, Immunoglobulins
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