By a News Reporter-Staff News Editor at Cancer Weekly -- Current study results on Immunology have been published. According to news originating from Lexington, Kentucky, by NewsRx correspondents, research stated, "The ingenious design of the bacterial virus phi29 DNA packaging nanomotor with an elegant and elaborate channel has inspired its application for single molecule detection of antigen/antibody interactions. The hub of this bacterial virus nanomotor is a truncated cone-shaped connector consisting of 12 protein subunits."
Our news journalists obtained a quote from the research from the University of Kentucky, "These subunits form a ring with a central 3.6-nm channel acting as a path for dsDNA to enter during packaging and to exit during infection. The connector has been inserted into a lipid bilayer. Herein, we reengineered an Epithelial Cell Adhesion Molecule (EpCAM) peptide into the C-terminal of nanopore as a probe to specifically detect EpCAM antibody (Ab) in nanomolar concentration at the single molecule level. The binding of Abs sequentially to each peptide probe induced stepwise blocks in current. The distinctive current signatures enabled us to analyze the docking and undocking kinetics of Ab-probe interactions and:Pen determine the K-d. The signal of EpCAM antibody can be discriminated from the background events in the presence of nonspecific antibody or serum."
According to the news editors, the research concluded: "Our results demonstrate the feasibility of generating a highly sensitive platform for detecting antibodies at extremely low concentrations in the presence of contaminants."
For more information on this research see: Engineered Nanopore of Phi29 DNA-Packaging Motor for Real-Time Detection of Single Colon Cancer Specific Antibody in Serum. ACS Nano, 2013;7(11):9814-9822. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Nano - www.pubs.acs.org/journal/ancac3)
The news correspondents report that additional information may be obtained from S.Y. Wang, University of Kentucky, Markey Canc Center, Lexington, KY 40536, United States. Additional authors for this research include F. Haque, P.G. Rychahou, B.M. Evers and P.X. Guo (see also Immunology).
Keywords for this news article include: Antibodies, Cancer, Kentucky, Oncology, Lexington, Viral DNA, Immunology, Engineering, DNA Research, United States, Blood Proteins, Immunoglobulins, North and Central America
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