By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Granulocytes. According to news reporting out of Melbourne, Australia, by NewsRx editors, research stated, "Neutrophilic granulocytes play a fundamental role in cardiovascular disease. They interact with platelet aggregates via the integrin Mac-1 and the platelet receptor glycoprotein Ib alpha (GPIb alpha)."
Our news journalists obtained a quote from the research from Monash University, "In vivo, GPIb alpha presentation is highly variable under different physiological and pathophysiological conditions. Here, we quantitatively determined the conditions for neutrophil adhesion in a biomimetic in vitro system, which allowed precise adjustment of the spacings between human GPlb alpha presented on the nanoscale from 60 to 200 nm. Unlike most conventional nanopatterning approaches, this method provided control over the local receptor density (spacing) rather than just the global receptor density. Under physiological flow conditions, neutrophils required a minimum spacing of GPlba molecules to successfully adhere. In contrast, under low-flow conditions, neutrophils adhered on all tested spacings with subtle but nonlinear differences in cell response, including spreading area, spreading kinetics, adhesion maturation, and mobility. Surprisingly, Mac-l-dependent neutrophil adhesion was very robust to GPlba density variations up to 1 order of magnitude. This complex response map indicates that neutrophil adhesion under flow and adhesion maturation are differentially regulated by GPlba density. Our study reveals how Mac-1/GPlba interactions govern cell adhesion and how neutrophils process the number of available surface receptors on the nanoscale."
According to the news editors, the research concluded: "In the future, such in vitro studies can be useful to determine optimum therapeutic ranges for targeting this interaction."
For more information on this research see: Adhesion Maturation of Neutrophils on Nanoscopically Presented Platelet Glycoprotein Ib alpha. ACS Nano, 2013;7(11):9984-9996. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Nano - www.pubs.acs.org/journal/ancac3)
Our news journalists report that additional information may be obtained by contacting S. Kruss, Monash University, Australian Center Blood Dis, Melbourne, Vic 3004, Australia. Additional authors for this research include L. Erpenbeck, K. Amschler, T.A. Mundinger, H. Boehm, H.J. Helms, T. Friede, R.K. Andrews, M.P. Schon and J.P. Spatz (see also Granulocytes).
Keywords for this news article include: Melbourne, Nanoscale, Immunology, Phagocytes, Blood Cells, Neutrophils, Granulocytes, Therapeutics, Glycoproteins, Nanotechnology, Glycoconjugates, Emerging Technologies, Hemic and Immune Systems, Australia and New Zealand
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