By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on Small Interference RNAs (siRNAs). According to news reporting originating from Seoul, South Korea, by NewsRx correspondents, research stated, "Transferrin (TF) is widely used as a tumor-targeting ligand for the delivery of anticancer drugs because the TF receptor is overexpressed on the surface of various fast-growing cancer cells. In this article, we report on TF nanoparticles as an siRNA delivery carrier for in vivo tumor-specific gene silencing."
Our news editors obtained a quote from the research from Korea University, "To produce siRNA carrying TF nanoparticles (NPs), both TF and siRNA were chemically modified with sulfhydryl groups that can build up self-crosslinked siRNA-TF NPs. Self-polymerized 5'-end thiol-modified siRNA (poly siRNA, psi) and thiolated transferrin (tTF) were spontaneously cross-linked to form stable NPs (psi-tTF NPs) under optimized conditions, and they could be reversibly degraded to release functional monomeric siRNA molecules under reductive conditions. Receptor-mediated endocytosis of TF induced rapid tumor-cell-specific uptake of the psi-tTF NPs, and the internalized NPs resulted in a downregulation of the target protein in red-fluorescent-protein-expressing melanoma cancer cells (RFP/B16F10) with negligible cytotoxicity. After systemic administration, the psi-tTF NPs showed marked accumulation at the tumor, leading to successful target-gene silencing in vivo."
According to the news editors, the research concluded: "This psi-tTF NP system provided a safe and effective strategy for in vivo systemic siRNA delivery for cancer therapy."
For more information on this research see: Tumor-Targeting Transferrin Nanoparticles for Systemic Polymerized siRNA Delivery in Tumor-Bearing Mice. Bioconjugate Chemistry, 2013;24(11):1850-1860. Bioconjugate Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Bioconjugate Chemistry - www.pubs.acs.org/journal/bcches)
The news editors report that additional information may be obtained by contacting J.Y. Yhee, Korea University, KU KIST Sch, Seoul 136701, South Korea. Additional authors for this research include S.J. Lee, S. Lee, S. Song, H.S. Min, S.W. Kang, S. Son, S.Y. Jeong, I.C. Kwon, S.H. Kim and K. Kim (see also Small Interference RNAs (siRNAs)).
Keywords for this news article include: Asia, Seoul, Cancer, Genetics, Oncology, South Korea, Transferrin, Nanoparticle, Beta-Globulins, Blood Proteins, Nanotechnology, Acute-Phase Proteins, Emerging Technologies, Iron-Binding Proteins, Small Interference RNAs (siRNAs)
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