By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Proteins have been presented. According to news reporting out of Frankfurt, Germany, by NewsRx editors, research stated, "Many questions concerning the molecular processes during biological aging remain unanswered. Since mitochondria are central players in aging, we applied quantitative two-dimensional difference gel electrophoresis (2D-DIGE) coupled to protein identification by mass spectrometry to study the age-dependent changes in the mitochondrial proteome of the fungus Podospora anserina -a well-established aging model. 67 gel spots exhibited significant, but remarkably moderate intensity changes."
Our news journalists obtained a quote from the research from Johann Wolfgang Goethe-University, "While typically the observed changes in protein abundance occurred progressively with age, for several proteins a pronounced change was observed at late age, sometimes inverting the trend observed at younger age. The identified proteins were assigned to a wide range of metabolic pathways including several implicated previously in biological aging. An overall decrease for subunits of complexes I and V of oxidative phosphorylation was confirmed by Western blot analysis and blue-native electrophoresis. Changes in several groups of proteins suggested a general increase in protein biosynthesis possibly reflecting a compensatory mechanism for increased quality control-related protein degradation at later age. Age-related augmentation in abundance of proteins involved in biosynthesis, folding, and protein degradation pathways sustain these observations. Furthermore, a significant decrease of two enzymes involved in the degradation of ?-aminobutyrate (GABA) supported its previously suggested involvement in biological aging. We have followed the time course of changes in protein abundance during aging of the fungus P. anserina."
According to the news editors, the research concluded: "The observed moderate but significant changes provide insight into the molecular adaptations to biological aging and highlight the metabolic pathways involved, thereby offering new leads for future research."
For more information on this research see: Age-related changes in the mitochondrial proteome of the fungus Podospora anserina analyzed by 2D-DIGE and LC-MS/MS. Journal of Proteomics, 2013;91():358-74. (Elsevier - www.elsevier.com; Journal of Proteomics - www.elsevier.com/wps/product/cws_home/713351)
Our news journalists report that additional information may be obtained by contacting M.A. Chimi, Molecular Bioenergetics Group, Medical School, Cluster of Excellence Frankfurt Macromolecular Complexes, Centre for Membrane Proteomics, Johann Wolfgang Goethe University, 60590 Frankfurt am Main, Germany. Additional authors for this research include S. Drose, I. Wittig, H. Heide, M. Steger, A. Werner, A. Hamann, H.D. Osiewacz and U. Brandt (see also Proteins).
Keywords for this news article include: Europe, Germany, Peptides, Proteins, Proteome, Frankfurt, Amino Acids.
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