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Researchers from Three Gorges University Discuss Findings in Enzymes and Coenzymes

January 24, 2014

By a News Reporter-Staff News Editor at Science Letter -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news reporting originating from Chongqing, People's Republic of China, by NewsRx correspondents, research stated, "A new and enzyme-free electrochemical immunoassay protocol was developed for the sensitive electronic monitoring of neuron-specific enolase (NSE) on a monoclonal mouse anti-human NSE antibody (mAb)-modified glassy carbon electrode, using guanine-decorated graphene nanostructures (GGN) as nanotags. To construct such an enzyme-free immunoassay format, guanine and polyclonal rabbit anti-human NSE antibody (pAb) were co-immobilized on the graphene nanostructures through the carbodiimide coupling."

Our news editors obtained a quote from the research from Three Gorges University, "Based on a sandwich-type immunoassay mode, the assay was carried out in 0.1 M pH 7.4 PBS containing 5 mu M Ru(bpy)(3)(2+) through the catalytic oxidation of Ru(bpy)32+ toward the guanine on the GGN. The presence of graphene nanostructures increased the immobilized amount of guanine, thus amplifying a detectable electronic signal. The covalent conjugation of guanine and pAb on the GGN resulted in a good repeatability and intermediate reproducibility down to 9.5%. Under optimal conditions, the dynamic concentration range of the developed immunoassay spanned from 0.005 to 80 ng mL(-1) NSE with a detection limit of 1.0 pg mL(-1) at the 3S(blank) level."

According to the news editors, the research concluded: "In addition, the methodology was evaluated by assaying the spiking serum samples, and the relative standard deviation (RSD) between the electrochemical immunoassay and a commercialized enzyme-linked immunosorbent assay (ELISA) were 2.8 - 7.0%."

For more information on this research see: Guanine-Decorated Graphene Nanostructures for Sensitive Monitoring of Neuron-Specific Enolase Based on an Enzyme-Free Electrocatalytic Reaction. Analytical Sciences, 2013;29(12):1195-1201. Analytical Sciences can be contacted at: Japan Soc Analytical Chemistry, 26-2 Nishigotanda 1 Chome Shinagawa-Ku, Tokyo, 141, Japan (see also Enzymes and Coenzymes).

The news editors report that additional information may be obtained by contacting G.Z. Li, Chongqing Three Gorges Univ, Dept. of Sports & Hlth, Chongqing 404100, People's Republic of China.

Keywords for this news article include: Asia, Antibodies, Chongqing, Chemistry, Immunology, Blood Proteins, Nanostructural, Nanostructures, Nanotechnology, Electrochemical, Immunoglobulins, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China

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Source: Science Letter

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