By a News Reporter-Staff News Editor at Science Letter -- Investigators publish new report on Enzymes and Coenzymes. According to news reporting out of Buenos Aires, Argentina, by NewsRx editors, research stated, "In this work, a novel molecularly imprinted polymer (MIP) for use as a solid phase extraction sorbent was developed for the determination of coenzyme Q10 (CoQ10) in liver extract. CoQ10 is an essential cofactor in mitochondrial oxidative phosphorylation and a powerful antioxidant agent found in low concentrations in biological samples."
Our news journalists obtained a quote from the research from the University of Buenos Aires, "This fact and its high hydrophobicity make the analysis of CoQ10 technically challenging. Accordingly, a MIP was synthesised using coenzyme Q0 as the template, methacrylic acid as the functional monomer, acetonitrile as the porogen, ethylene glycol dimethacrylate as the crosslinker and benzoyl peroxide as the initiator. Various parameters affecting the polymer preparation and extraction efficiency were evaluated. Morphological characterisation of the MIP and its proper comparison with C18 as a sorbent in solid phase extraction were performed. The optimal conditions for the molecularly imprinted solid phase extraction (MISPE) consisted of 400 mu L of sample mixed with 30 mg of MIP and 600 mu L of water to reach the optimum solution loading. The loading was followed by a washing step consisting of 1 mL of a 1-propanol solution (1-propanol: water, 30:70, v/v) and elution with 1 mL of 1-propanol. After clean-up, the CoQ10 in the samples was analysed by high performance liquid chromatography. The extraction recoveries were higher than 73.7% with good precision (3.6-8.3%). The limits of detection and quantification were 2.4 and 7.5 mu g g(-1), respectively, and a linear range between 7.5 and 150 mu g g(-1) of tissue was achieved."
According to the news editors, the research concluded: "The new MISPE procedure provided a successful clean-up for the determination of CoQ10 in a complex matrix."
For more information on this research see: The use of coenzyme Q0 as a template in the development of a molecularly imprinted polymer for the selective recognition of coenzyme Q10. Analytica Chimica Acta, 2014;807():67-74. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
Our news journalists report that additional information may be obtained by contacting M. Contin, University of Buenos Aires, Fac Pharm & Biochem, Dept. of Pharmaceut Technol, RA-1113 Buenos Aires, DF, Argentina. Additional authors for this research include S. Flor, M. Martinefski, S. Lucangioli and V. Tripodi (see also Enzymes and Coenzymes).
Keywords for this news article include: Argentina, 1-Propanol, Buenos Aires, South America, Enzymes and Coenzymes
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