By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Biotechnology have been presented. According to news reporting from Faro, Portugal, by NewsRx journalists, research stated, "Natural products are considered an extremely valuable source for the discovery of new drugs against diverse pathologies. As yet, we have only explored a fraction of the diversity of bioactive compounds, and opportunities for discovering new natural products leading to new drugs are huge."
The news correspondents obtained a quote from the research from the University of Algarve, "In the present study, U2nesRELOC, a previously established cell-based imaging assay, was employed to screen a collection of extracts of microbial origin for nuclear export inhibition activity. The fluorescent signal of untreated U2nesRELOC cells localizes predominantly to the cytoplasm. Upon treatment with the nuclear export inhibitor leptomycin B, the fluorescent-tagged reporter proteins appear as speckles in the nucleus. A proprietary collection of extracts from fungi, actinomycetes, and unicellular bacteria that covers an uncommonly broad chemical space was used to interrogate this nuclear export assay system. A two-step image-based analysis allowed us to identify 12 extracts with biological activities that are not associated with previously known active metabolites. The fractionation and structural elucidation of active compounds revealed several chemical structures with nuclear export inhibition activity. Here we show that substrates of the nuclear export receptor CRM1, such as Rev, FOXO3a and NF-B, accumulate in the nucleus in the presence of the fungal metabolite MDN-0105 with an IC50 value of 3.4 mu M. Many important processes in tumor formation and progression, as well as in many viral infections, critically depend on the nucleocytoplasmic trafficking of proteins and RNA molecules. Therefore, the disruption of nuclear export is emerging as a novel therapeutic approach with enormous clinical potential."
According to the news reporters, the research concluded: "Our work highlights the potential of applying high-throughput phenotypic imaging on natural product extracts to identify novel nuclear export inhibitors."
For more information on this research see: High-Content Screening of Natural Products Reveals Novel Nuclear Export Inhibitors. Journal of Biomolecular Screening, 2014;19(1):57-65. Journal of Biomolecular Screening can be contacted at: Sage Publications Inc, 2455 Teller Rd, Thousand Oaks, CA 91320, USA. (Sage Publications - www.sagepub.com/; Journal of Biomolecular Screening - jbx.sagepub.com)
Our news journalists report that additional information may be obtained by contacting B. Cautain, University of Algarve, Center Biomed Mol & Estrutural, IBB, Faro, Portugal. Additional authors for this research include N. de Pedro, V.M. Garzon, M.M. de Escalona, V.G. Menendez, J.R. Tormo, J. Martin, N. El Aouad, F. Reyes, F. Asensio, O. Genilloud, F. Vicente and W. Link (see also Biotechnology).
Keywords for this news article include: Faro, Biotechnology, Europe, Portugal
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