By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Biomaterials. According to news reporting originating from Karlsruhe, Germany, by NewsRx correspondents, research stated, "Transmigrating cells often need to deform cell body and nucleus to pass through micrometer-sized pores in extracellular matrix scaffolds. Furthermore, chemoattractive signals typically guide transmigration, but the precise interplay between mechanical constraints and signaling mechanisms during 3D matrix invasion is incompletely understood and may differ between cell types."
Our news editors obtained a quote from the research from the Karlsruhe Institute of Technology, "Here, we used Direct Laser Writing to fabricate 3D cell culture scaffolds with adjustable pore sizes (2-10 mu m) on a microporous carrier membrane for applying diffusible chemical gradients. Mouse embryonic fibroblasts invade 10 gm pore scaffolds even in absence of chemoattractant, but invasion is significantly enhanced by knockout of lamin A/C, a known regulator of cell nucleus stiffness. Nuclear stiffness thus constitutes a major obstacle to matrix invasion for fibroblasts, but chemotaxis signals are not essential. In contrast, epithelial A549 cells do not enter 10 mu m pores even when lamin A/C levels are reduced, but readily enter scaffolds with pores down to 7 mu m in presence of chemoattractant (serum). Nuclear stiffness is therefore not a prime regulator of matrix invasion in epithelial cells, which instead require chemoattractive signals."
According to the news editors, the research concluded: "Micro-structured scaffolds with adjustable pore size and diffusible chemical gradients are thus a valuable tool to dissect cell-type specific mechanical and signaling aspects during matrix invasion."
For more information on this research see: Multifunctional polymer scaffolds with adjustable pore size and chemoattractant gradients for studying cell matrix invasion. Biomaterials, 2014;35(2):611-619. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
The news editors report that additional information may be obtained by contacting A.M. Greiner, Karlsruhe Inst Technol, DFG Center Funct Nanostruct CFN, D-76131 Karlsruhe, Germany. Additional authors for this research include M. Jackel, A.C. Scheiwe, D.R. Stamow, T.J. Autenrieth, J. Lahann, C.M. Franz and M. Bastmeyer (see also Biomaterials).
Keywords for this news article include: Europe, Germany, Karlsruhe, Biomaterials
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