By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Life Science Research. According to news reporting out of Chapel Hill, North Carolina, by NewsRx editors, research stated, "One of the most fundamental concepts of microscopy is that of resolution-the ability to clearly distinguish two objects as separate. Recent advances such as structured illumination microscopy (SIM) and point localization techniques including photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM) strive to overcome the inherent limits of resolution of the modern light microscope."
Our news journalists obtained a quote from the research from the University of North Carolina, "These techniques, however, are not always feasible or optimal for live cell imaging. Thus, in this review, we explore three techniques for extracting high resolution data from images acquired on a widefield microscope-deconvolution, model convolution, and Gaussian fitting. Deconvolution is a powerful tool for restoring a blurred image using knowledge of the point spread function (PSF) describing the blurring of light by the microscope, although care must be taken to ensure accuracy of subsequent quantitative analysis. The process of model convolution also requires knowledge of the PSF to blur a simulated image which can then be compared to the experimentally acquired data to reach conclusions regarding its geometry and fluorophore distribution. Gaussian fitting is the basis for point localization microscopy, and can also be applied to tracking spot motion over time or measuring spot shape and size."
According to the news editors, the research concluded: "All together, these three methods serve as powerful tools for high-resolution imaging using widefield microscopy. J. Cell. Physiol. 229: 132-138, 2014."
For more information on this research see: Bending the Rules: Widefield Microscopy and the Abbe Limit of Resolution. Journal of Cellular Physiology, 2014;229(2):132-138. Journal of Cellular Physiology can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Journal of Cellular Physiology - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-4652)
Our news journalists report that additional information may be obtained by contacting J.S. Verdaasdonk, University of North Carolina, Dept. of Biol, Chapel Hill, NC 27599, United States. Additional authors for this research include A.D. Stephens, J. Haase and K. Bloom (see also Life Science Research).
Keywords for this news article include: Chapel Hill, United States, North Carolina, Life Science Research, North and Central America
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