By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Fibroblasts. According to news reporting from Brussels, Belgium, by NewsRx journalists, research stated, "Carbon nanotubes (CNT) can induce lung inflammation and fibrosis in rodents. Several studies have identified the capacity of CNT to stimulate the proliferation of fibroblasts."
The news correspondents obtained a quote from the research from the Catholic University of Louvain, "We developed and validated experimentally here a simple and rapid in vitro assay to evaluate the capacity of a nanomaterial to exert a direct pro-fibrotic effect on fibroblasts. The activity of several multi-wall (MW) CNT samples (NM400, the crushed form of NM400 named NM400c, NM402 and MWCNTg 2400) and asbestos (crocidolite) was investigated in vitro and in vivo. The proliferative response to MWCNT was assessed on mouse primary lung fibroblasts, human fetal lung fibroblasts (HFL-1), mouse embryonic fibroblasts (BALB-3T3) and mouse lung fibroblasts (MLg) by using different assays (cell counting, WST-1 assay and propidium iodide PI staining) and dispersion media (fetal bovine serum, FBS and bovine serum albumin, BSA). C57BL/6 mice were pharyngeally aspirated with the same materials and lung fibrosis was assessed after 2 months by histopathology, quantification of total collagen lung content and pro-fibrotic cytokines in broncho-alveolar lavage fluid (BALF). MWCNT (NM400 and NM402) directly stimulated fibroblast proliferation in vitro in a dose-dependent manner and induced lung fibrosis in vivo. NM400 stimulated the proliferation of all tested fibroblast types, independently of FBS- or BSA- dispersion. Results obtained by WST1 cell activity were confirmed with cell counting and cell cycle (PI staining) assays. Crocidolite also stimulated fibroblast proliferation and induced pulmonary fibrosis, although to a lesser extent than NM400 and NM402. In contrast, shorter CNT (NM400c and MWCNTg 2400) did not induce any fibroblast proliferation or collagen accumulation in vivo, supporting the idea that CNT structure is an important parameter for inducing lung fibrosis. In this study, an optimized proliferation assay using BSA as a dispersant, MLg cells as targets and an adaptation of WST-1 as readout was developed."
According to the news reporters, the research concluded: "The activity of MWCNT in this test strongly reflects their fibrotic activity in vivo, supporting the predictive value of this in vitro assay in terms of lung fibrosis potential."
For more information on this research see: Towards predicting the lung fibrogenic activity of nanomaterials: experimental validation of an in vitro fibroblast proliferation assay. Particle and Fibre Toxicology, 2013;10():1-14. Particle and Fibre Toxicology can be contacted at: Biomed Central Ltd, 236 Grays Inn Rd, Floor 6, London WC1X 8HL, England. (BioMed Central - www.biomedcentral.com/; Particle and Fibre Toxicology - www.particleandfibretoxicology.com)
Our news journalists report that additional information may be obtained by contacting G. Vietti, Catholic University of Louvain, De Duve Inst, B-1200 Brussels, Belgium. Additional authors for this research include S. Ibouraadaten, M. Palmai-Pallag, Y. Yakoub, C. Bailly, I. Fenoglio, E. Marbaix, D. Lison and S. van den Brule (see also Fibroblasts).
Keywords for this news article include: Europe, Belgium, Brussels, Fibroblasts, Nanomaterial, Nanotechnology, Bovine Serum Albumin, Emerging Technologies, Connective Tissue Cells
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