By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on DNA Research have been published. According to news reporting from Jiangsu, People's Republic of China, by NewsRx journalists, research stated, "Nanopore-based sensor is a new single-molecule level sensing method, which involves detecting the current change and translocation time of the molecule when it passes through the pore by the electrophoresis. In this work, the lambda-DNA was driven thread through the solid-state nanopore of different diameters to characterize DNA's physical properties from its translocation process in 1 M KCl salt solution."
The news correspondents obtained a quote from the research from Southeast University, "Firstly, the frequency of events increased with the rising voltages. We found that the event frequency for 200 mV decreased because of the reduced concentration of the lambda-DNA after about two hours' translocation process under other voltages. And the duration times of lambda-DNA translocation events which linearly passed through the nanopore at different voltages were analyzed with the first-passage-time distribution indicated that the electrophoretic mobility mu of lambda-DNA is 1.153 x 10(-10) m(2) V-1 mu s(-1) under the condition of 1 M KCl buffer and 22 nm nanopore. Besides, we investigated two methods to calculate the diffusion constant D. The two results agreed with each other quite well. The capture rate increased with the voltage at a fixed DNA concentration. The data analysis of DNA passing through nanopores with different sizes suggested that the diameter of the nanopore plays a key factor in DNA translocation behavior."
According to the news reporters, the research concluded: "This is an important result for the label-free sensing by solid-state nanopore in the future and will provide more evidences for the development of nanopores as a multi-functional sensor for a wide range of biopolymers and nanoparticles."
For more information on this research see: Voltage Dependence Resistive Pulse of Lambda-DNA Translocation with Different Size Solid-State Nanopore Sensor. Science of Advanced Materials, 2013;5(12):2032-2038. Science of Advanced Materials can be contacted at: Amer Scientific Publishers, 26650 The Old Rd, Ste 208, Valencia, CA 91381-0751, USA. (American Scientific Publishers - www.aspbs.com/)
Our news journalists report that additional information may be obtained by contacting L.P. Liu, Southeast Univ, SEU FEI Nanopico Center, Key Lab MEMS, Minist Educ, Nanjing 210096, Jiangsu, People's Republic of China. Additional authors for this research include X. Xie, J.L. Kong, H.W. Wu and Q.J. Liu (see also DNA Research).
Keywords for this news article include: Asia, Jiangsu, DNA Research, People's Republic of China
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