By a News Reporter-Staff News Editor at Science Letter -- A new study on Immunology is now available. According to news reporting originating from Fukuoka, Japan, by NewsRx correspondents, research stated, "We attempted to evaluate a novel purification method of immunoglobulins (IgGs) by using a mutant type of protein A. Although this mutant protein A binds to IgGs at 5 degrees C, the IgGs are released at 40 degrees C; hence, it was designated as thermo-responsive protein A (TRPA). We aimed to purify IgG1 from the culture supernatant of CHO cells producing AE6F4 human monoclonal IgG1."
Our news editors obtained a quote from the research from Kyushu University, "AE6F4 IgG1 was purified using only a TRPA-filled column and by modifying the temperature, without any exposure to acidic conditions. Furthermore, the purified AE6F4 IgG1 maintained the inherent binding affinity to antigen, while this property was lost in AE6F4 IgG1 purified using a conventional protein A (CPA) column possibly because of product aggregation and fragmentation. These data suggest that IgG is sensitive to acid treatment; however, it can be highly purified with retention of high affinity by using a TRPA column."
According to the news editors, the research concluded: "Further, this purification method can be used on an industrial scale for the purification of antibody drugs."
For more information on this research see: Novel purification method of human immunoglobulin by using a thermo-responsive protein A. Journal of Chromatography A, 2013;1305():149-153. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)
The news editors report that additional information may be obtained by contacting I. Koguma, Kyushu University, Fac Agr, Higashi Ku, Fukuoka 8128581, Japan. Additional authors for this research include S. Yamashita, S. Sato, K. Okuyama and Y. Katakura (see also Immunology).
Keywords for this news article include: Asia, Japan, Fukuoka, Immunology, Immunoproteins, Immunoglobulins, Serum Globulins
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