By a News Reporter-Staff News Editor at Science Letter -- Researchers detail new data in Immunology. According to news reporting originating in Rzeszow, Poland, by NewsRx journalists, research stated, "Dynamics of the purification process of a CHO derived monoclonal antibody by ion exchange chromatography (IEC), hydrophobic interaction chromatography CHIC) and their integration has been investigated. To quantify the adsorption behavior of the target protein (IgG1) and impurities contained in the supernatant, their elution course on IEC and HIC columns has been analyzed versus pH and/or the salt concentration in the mobile phase."
The news reporters obtained a quote from the research from the Rzeszow University of Technology, "A short-cut method has been proposed for mathematical modeling and determining underlying kinetic and thermodynamic parameters. The accuracy of the model predictions has been verified by comparing the simulated and experimental band profiles recorded in both chromatographic processes. After verification, the model was used to optimize operating conditions for the column loading and chromatographic elution in the integrated process IEC/HIC. Two alternative loading techniques based on the upstream and downstream feed dilution were taken into account in the optimization routine. In the first one the feed stream was diluted with the loading buffer prior to the column loading, while in the latter one the feed dilution was realized inside the column using the multiple-injection technique."
According to the news reporters, the research concluded: "It was shown that the downstream dilution allowed significant reduction of the contact time between the protein and the loading buffer."
For more information on this research see: Isolation of monoclonal antibody from a Chinese hamster ovary supernatant. II: Dynamics of the integrated separation on ion exchange and hydrophobic interaction chromatography media. Journal of Chromatography A, 2013;1305():64-75. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)
Our news correspondents report that additional information may be obtained by contacting W. Marek, Rzeszow Univ Technol, Chem & Proc Engn Department, PL-35959 Rzeszow, Poland. Additional authors for this research include R. Muca, S. Wos, W. Piatkowski and D. Antos (see also Immunology).
Keywords for this news article include: Antibodies, Poland, Europe, Rzeszow, Immunology, Blood Proteins, Immunoglobulins
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