By a News Reporter-Staff News Editor at Science Letter -- Investigators publish new report on Chromatographic Science. According to news reporting originating from Daejeon, South Korea, by NewsRx correspondents, research stated, "A method for assaying a novel phosphodiesterase-4 inhibitor, 2-aryl-7(3',4'-dialkoxyphenyl)-pyrazolo [1,5-alpha] pyrimidine (PDE-310), was developed and validated in rat plasma using liquid chromatography-tandem mass spectrometry (LC-MS-MS). Rat plasma samples were processed by liquid-liquid extraction with ethyl acetate and injected onto the LC-MS-MS system for quantification."
Our news editors obtained a quote from the research from the Korea Research Institute of Chemical Technology, "PDE-310 and imipramine (i.e., internal standard) were separated using a Gemini C18 column with mixture of acetonitrile and 0.1% formic acid (70:30, v/v) as the mobile phase. The ion transitions monitored were m/z 425.0 ? 331.0 for PDE-310 and m/z 281.3 ? 86.1 for imipramine in the multiple-reaction monitoring mode. The detector response was specific and linear for PDE-310 concentrations in the range of 0.1-50 g/mL. The intra-day and inter-day precision and accuracy of the method were determined to be within the acceptance criteria for assay validation guidelines. The recoveries were approximately 85.7 and 88.2% from rat plasma for PDE-310 and imipramine, respectively. PDE-310 was stable under various processing and handling conditions."
According to the news editors, the research concluded: "PDE-310 concentrations were readily measured in rat plasma samples up to 8 h after an intravenous administration of PDE-310, suggesting that the assay is practically useful."
For more information on this research see: Determination of 2-aryl-7(3',4'-dialkoxyphenyl)-pyrazolo [1,5-alpha] pyrimidine, a novel phosphodiesterase-4 inhibitor, in rat plasma by liquid chromatography-tandem mass spectrometry. Journal of Chromatographic Science, 2013;51(6):517-23. (Oxford University Press - www.oup.com/; Journal of Chromatographic Science - chromsci.oxfordjournals.org)
The news editors report that additional information may be obtained by contacting K.R. Lee, Drug Discovery Platform Technology Team, Korea Research Institute of Chemical Technology, Daejeon 305-600, South Korea. Additional authors for this research include J.S. Song, Y.J. Chae, M.S. Kim, S.H. Choi, D.J. Jeon, M.A. Bae and S.H Ahn (see also Chromatographic Science).
Keywords for this news article include: Asia, Daejeon, South Korea, Chromatographic Science.
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