By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Immunology. According to news reporting originating in Lisbon, Portugal, by NewsRx journalists, research stated, "Monoclonal antibodies (mAbs) are important therapeutic proteins. One of the challenges facing large-scale production of monoclonal antibodies is the capacity bottleneck in downstream processing, which can be circumvented by using magnetic stimuli-responsive polymer nanoparticles."
The news reporters obtained a quote from the research from Technical University, "In this work, stimuli-responsive magnetic particles composed of a magnetic poly(methyl methacrylate) core with a poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-co-AA)) shell cross-linked with N, N'-methylenebisacrylamide were prepared by miniemulsion polymerization. The particles were shown to have an average hydrodynamic diameter of 317 nm at 18°C, which decreased to 277 nm at 41°C due to the collapse of the thermo-responsive shell. The particles were superparamagnetic in behavior and exhibited a saturation magnetization of 12.6 emu/g. Subsequently, we evaluated the potential of these negatively charged stimuli-responsive magnetic particles in the purification of a monoclonal antibody from a diafiltered CHO cell culture supernatant by cation exchange. The adsorption of antibodies onto P(NIPAM-co-AA)-coated nanoparticles was highly selective and allowed for the recovery of approximately 94% of the mAb. Different elution strategies were employed providing highly pure mAb fractions with host cell protein (HCP) removal greater than 98%."
According to the news reporters, the research concluded: "By exploring the stimuli-responsive properties of the particles, shorter magnetic separation times were possible without significant differences in product yield and purity."
For more information on this research see: Stimuli-responsive magnetic nanoparticles for monoclonal antibody purification. Biotechnology Journal, 2013;8(6):709-17. (Wiley-Blackwell - www.wiley.com/; Biotechnology Journal - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314)
Our news correspondents report that additional information may be obtained by contacting L. Borlido, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Dept. of Bioengineering, Instituto Superior Tecnico, Technical University of Lisbon, 1049-001 Lisbon, Portugal. Additional authors for this research include L. Moura, A.M. Azevedo, A.C. Roque, M.R. Aires-Barros and J.P Farinha (see also Immunology).
Keywords for this news article include: Antibodies, Lisbon, Europe, Therapy, Portugal, Immunology, Nanoparticle, Blood Proteins, Nanotechnology, Immunoglobulins, Emerging Technologies.
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