By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Technology. According to news reporting originating from Sao Paulo, Brazil, by NewsRx correspondents, research stated, "Recent progress in understanding the molecular basis of autophagy has demonstrated its importance in several areas of human health. Affordable screening techniques with higher sensitivity and specificity to identify autophagy are, however, needed to move the field forward."
Our news editors obtained a quote from the research from the University of Sao Paulo, "In fact, only laborious and/or expensive methodologies such as electron microscopy, dye-staining of autophagic vesicles, and LC3-II immunoblotting or immunoassaying are available for autophagy identification. Aiming to fulfill this technical gap, we describe here the association of three widely used assays to determine cell viability -Crystal Violet staining (CVS), 3-[4, 5-dimethylthiaolyl]-2, 5-diphenyl-tetrazolium bromide (MTT) reduction, and neutral red uptake (NRU) -to predict autophagic cell death in vitro. The conceptual framework of the method is the superior uptake of NR in cells engaging in autophagy. NRU was then weighted by the average of MTT reduction and CVS allowing the calculation of autophagic arbitrary units (AAU), a numeric variable that correlated specifically with the autophagic cell death."
According to the news editors, the research concluded: "The proposed strategy is very useful for drug discovery, allowing the investigation of potential autophagic inductor agents through a rapid screening using mammalian cell lines B16-F10, HaCaT, HeLa, MES-SA, and MES-SA/Dx5 in a unique single microplate."
For more information on this research see: Rapid screening of potential autophagic inductor agents using mammalian cell lines. Biotechnology Journal, 2013;8(6):730-7. (Wiley-Blackwell - www.wiley.com/; Biotechnology Journal - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314)
The news editors report that additional information may be obtained by contacting W.K. Martins, Dept. of Biochemistry, University of Sao Paulo, Sao Paulo, Brazil. Additional authors for this research include D. Severino, C. Souza, B.S. Stolf and M.S Baptista (see also Technology).
Keywords for this news article include: Brazil, Sao Paulo, Technology, South America.
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