By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Technology. According to news reporting out of Hamburg, Germany, by NewsRx editors, research stated, "The identification of optimal expression conditions for state-of-the-art production of pharmaceutical proteins is a very time-consuming and expensive process. In this report a method for rapid and reproducible optimization of protein expression in an in-house designed small-scale BIOSTAT® multi-bioreactor plant is described."
Our news journalists obtained a quote from the research from the University of Applied Sciences, "A newly developed BioPAT® MFCS/win Design of Experiments (DoE) module (Sartorius Stedim Systems, Germany) connects the process control system MFCS/win and the DoE software MODDE® (Umetrics AB, Sweden) and enables therefore the implementation of fully automated optimization procedures. As a proof of concept, a commercial Pichia pastoris strain KM71H has been transformed for the expression of potential malaria vaccines. This approach has allowed a doubling of intact protein secretion productivity due to the DoE optimization procedure compared to initial cultivation results. In a next step, robustness regarding the sensitivity to process parameter variability has been proven around the determined optimum. Thereby, a pharmaceutical production process that is significantly improved within seven 24-hour cultivation cycles was established."
According to the news editors, the research concluded: "Specifically, regarding the regulatory demands pointed out in the process analytical technology (PAT) initiative of the United States Food and Drug Administration (FDA), the combination of a highly instrumented, fully automated multi-bioreactor platform with proper cultivation strategies and extended DoE software solutions opens up promising benefits and opportunities for pharmaceutical protein production."
For more information on this research see: Designing a fully automated multi-bioreactor plant for fast DoE optimization of pharmaceutical protein production. Biotechnology Journal, 2013;8(6):738-47. (Wiley-Blackwell - www.wiley.com/; Biotechnology Journal - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314)
Our news journalists report that additional information may be obtained by contacting J. Fricke, HAW-Hamburg University of Applied Sciences, D-21033 Hamburg, Germany. Additional authors for this research include K. Pohlmann, N.A. Jonescheit, A. Ellert, B. Joksch and R. Luttmann (see also Technology).
Keywords for this news article include: Europe, Hamburg, Germany, Software, Technology.
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