By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Gram-Positive Bacteria. According to news reporting originating from Jiangsu, People's Republic of China, by NewsRx correspondents, research stated, "Poly(epsilon-l-lysine) (epsilon-PL) producer strain Streptomyces albulus PD-1 secreted a novel polymeric substance into its culture broth along with epsilon-PL. The polymeric substance was purified to homogeneity and identified."
Our news editors obtained a quote from the research from the Nanjing University of Technology, "Matrix-assisted laser desorption ionization-time of flight mass spectrometry and nuclear magnetic resonance spectroscopy as well as other analytical techniques revealed that the substance was poly(l-diaminopropionic acid) (PDAP). PDAP is an l-alpha,beta-diaminopropionic acid oligomer linking between amino and carboxylic acid functional groups. The molecular weight of PDAP ranged from 500 to 1500 Da, and no co-polymers composed of l-diaminopropionic acid and l-lysine were present in the culture broth. Compared with epsilon-PL, PDAP exhibited stronger inhibitory activities against yeasts but weaker activities against bacteria. epsilon-PL and PDAP co-production was also investigated. Both epsilon-PL and PDAP were synthesized during the stationary phase of growth, and the final epsilon-PL and PDAP concentration reached 21.7 and 4.8 g L-1, respectively, in fed-batch fermentation. Citric acid feeding resulted in a maximum epsilon-PL concentration of 26.1 g L-1 and a decrease in the final concentration of PDAP to 3.8 g L-1."
According to the news editors, the research concluded: "No studies on epsilon-PL and PDAP co-production in Streptomyces albulus have been reported previously, and inhibition of by-products such as PDAP is potentially useful in epsilon-PL production."
For more information on this research see: Poly(l-diaminopropionic acid), a novel non-proteinic amino acid oligomer co-produced with poly(epsilon-l-lysine) by Streptomyces albulus PD-1. Applied Microbiology and Biotechnology, 2013;97(17):7597-7605. Applied Microbiology and Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Applied Microbiology and Biotechnology - www.springerlink.com/content/0175-7598/)
The news editors report that additional information may be obtained by contacting J. Xia, Nanjing Univ Technol, Coll Food Sci & Light Ind, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Jiangsu, People's Republic of China. Additional authors for this research include H. Xu, X.H. Feng, Z.X. Xu and B. Chi (see also Gram-Positive Bacteria).
Keywords for this news article include: Asia, Lysine, Jiangsu, Peptides, Proteins, Actinomycetales, Basic Amino Acids, Streptomycetaceae, Diamino Amino Acids, Essential Amino Acids, Gram-Positive Bacteria, People's Republic of China, Gram-Positive Endospore-Forming Rods
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC