By a News Reporter-Staff News Editor at Biotech Business Week -- Investigators discuss new findings in Genetics. According to news reporting out of Beijing, People's Republic of China, by NewsRx editors, research stated, "To identify novel small compound inhibitor of p53 protein. Mouse embryonic fibroblasts (MEF) and mouse embryonic stem (ES) cells were tested."
Our news journalists obtained a quote from the research from the Institute of Genetics and Developmental Biology, "Cell proliferation rate was determined using a Cell Proliferation Kit. The mRNA and protein levels of p53-related genes were measured using real-time PCR and Western blotting, respectively. Global response in the p53 signaling network was analyzed using Illumina whole-genome expression BeadChips. Treatment of MEF cells with a small molecule 1,4-bis-[4-(3-phenoxy-propoxy)-but-2-ynyl]-piperazine (G5) at 10 ?mol/L for 24 h markedly reduced the mRNA and protein levels of the p53 downstream genes MDM2 and p21. In G5-treated ES cells, a total of 372 differentially expressed genes were identified, and 18 among them were direct downstream genes of p53; 6 out of 9 p53-repressed genes were upregulated, and 5 out of 9 p53-activated genes were downregulated. In both MEF cells and ES cells, treatment of with G5 (10 ?mol/L) up to 48 h neither affected the proliferation rate nor caused morphological alterations."
According to the news editors, the research concluded: "G5 inhibits p53 activity and simultaneously preserves the normal growth and proliferation of cells, therefore is a new compound for studies of p53-mediated cell manipulation."
For more information on this research see: Identification of a small molecule 1,4-bis-[4-(3-phenoxy-propoxy)-but-2-ynyl]-piperazine as a novel inhibitor of the transcription factor p53. Acta Pharmacologica Sinica, 2013;34(6):805-10. (Nature Publishing Group - www.nature.com/; Acta Pharmacologica Sinica - www.nature.com/aps/)
Our news journalists report that additional information may be obtained by contacting X. Liu, Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Taiwan. Additional authors for this research include Y. Zhang, M. Tong, X.Y. Liu, G.Z. Luo, D.F. Xie, S.F. Ren, D.H. Bai, L. Wang, Q. Zhou and X.J Wang (see also Genetics).
Keywords for this news article include: Asia, Beijing, Genetics, p53 Gene, Treatment, Transcription Factors, People's Republic of China.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC