By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in DNA Research. According to news reporting originating in Fukuoka, Japan, by NewsRx journalists, research stated, "Localized and sustained delivery of the therapeutic genes using a solid carrier matrix is a potential approach to develop highly curative treatments. Electrospun nanofiber mesh of biodegradable polymer has been applied extensively as a carrier for localized and sustained delivery of drugs, proteins, and DNA, but it remains difficult to release sufficient amounts of DNA while maintaining structural and functional integrity."
The news reporters obtained a quote from the research from Kyushu University, "To realize the stable sustained release of the healthy plasmid DNA (pDNA) from electrospun fiber mesh, a novel method was examined for loading pDNA into the fibers based on solid-in-oil (S/O) nanodispersion of pDNA in organic solvent for electrospinning polymer solution: S/O nanodispersion electrospinning. A prepared pDNA-loaded fiber mesh made of biodegradable polymer showed sustained release of pDNA without burst release. From luciferase activity-based in vitro transcription-translation assay, pDNA released from meshes of the S/O nanodispersion retained about 60% luciferase activity of control pDNA, whereas pDNA released from the meshes of simple mixing showed only about 5% activity, indicating that S/O nanodispersion electrospinning is effective for loading pDNA into electrospun fiber meshes while maintaining their healthy functions."
According to the news reporters, the research concluded: "Effectiveness of S/O nanodispersion electrospinning was verified for fabricating a sustained release carrier matrix for high molecular weight bioactives, including therapeutic genes."
For more information on this research see: S/O-nanodispersion electrospun fiber mesh effective for sustained release of healthy plasmid DNA with the structural and functional integrity. Journal of Biomaterials Science Polymer Edition, 2013;24(10):1277-90 (see also DNA Research).
Our news correspondents report that additional information may be obtained by contacting T. Okuda, Division of Biomolecular Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan. Additional authors for this research include Y. Tahara, N. Kamiya, M. Goto and S. Kidoaki.
Keywords for this news article include: Asia, Japan, Fukuoka, DNA Research.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC