By a News Reporter-Staff News Editor at Pain & Central Nervous System Week -- Data detailed on Proteins have been presented. According to news originating from Gottingen, Germany, by NewsRx correspondents, research stated, "Alpha-synuclein (-syn) is the major component of Lewy bodies, a pathological hallmark of Parkinson's disease and other synucleinopathies. The characterization of -syn post-translational modifications (PTMs), thought to interfere with its aggregation propensity and cellular signaling, has been limited by the availability of extraction methods of endogenous protein from cells and tissues, and by the availability of antibodies toward -syn PTMs."
Our news journalists obtained a quote from the research from University Medical Center, "Here, by taking advantage of -syn thermostability, we applied a method to achieve high enrichment of soluble -syn both from cultured cells and brain tissues followed by proteomics analysis. Using this approach, we obtained 98% -syn sequence coverage in a variety of model systems, including a transgenic mouse model of PD, and validated the strategy by identifying previously described PTMs such as phosphorylation and N-terminal acetylation. Our findings demonstrate that this procedure overcomes existing technical limitations and can be used to facilitate the systematic study of -syn PTMs, thereby enabling the clarification of their role under physiological and pathological conditions."
According to the news editors, the research concluded: "Ultimately, this approach may enable the development of novel biomarkers and strategies for therapeutic intervention in synucleinopathies."
For more information on this research see: Heat-mediated enrichment of -synuclein from cells and tissue for assessing post-translational modifications. Journal of Neurochemistry, 2013;126(5):673-684. Journal of Neurochemistry can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Journal of Neurochemistry - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1471-4159)
The news correspondents report that additional information may be obtained from H.V. Miranda, Univ Med Center Gottingen, Center Nanoscale Microscopy & Mol Physiol Brain CNMP, Dept. of Neurodegenerat & Restorat Res, Gottingen, Germany. Additional authors for this research include W. Xiang, R.M. de Oliveira, T. Simoes, J. Pimentel, J. Klucken, D. Penque and T.F. Outeiro (see also Proteins).
Keywords for this news article include: Europe, Germany, Gottingen, Synucleins, Nerve Tissue Proteins
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