By a News Reporter-Staff News Editor at AIDS Weekly -- Fresh data on Immune System Diseases and Conditions are presented in a new report. According to news reporting out of Pittsburgh, Pennsylvania, by NewsRx editors, research stated, "Targeting protein-protein interactions (PPI) is an emerging field in drug discovery. Dimerization and PPI are essential properties of human immunodeficiency virus (HIV)-1 proteins, their mediated functions, and virus biology."
Our news journalists obtained a quote from the research from the University of Pittsburgh, "Additionally, dimerization is required for the functional interaction of HIV-1 proteins with many host cellular components. In this study, a bimolecular fluorescence complementation (BiFC)-based screening assay was developed that can quantify changes in dimerization, using HIV-1 viral protein R (Vpr) dimerization as a 'proof of concept.' Results demonstrated that Venus Vpr (generated by BiFC Vpr constructs) could be competed off in a dose-dependent manner using untagged, full-length Vpr as a competitor molecule. The change in signal intensity was measured quantitatively through flow cytometry and fluorescence microscopy in a high content screening assay. High content imaging was used to screen a library of small molecules for an effect on Vpr dimerization."
According to the news editors, the research concluded: "Among the tested molecules, a few of the small molecules demonstrate an effect on Vpr dimerization in a dose-dependent manner."
For more information on this research see: Development of a robust cell-based high-throughput screening assay to identify targets of HIV-1 viral protein R dimerization. Drug Design, Development and Therapy, 2013;7():403-12 (see also Immune System Diseases and Conditions).
Our news journalists report that additional information may be obtained by contacting C. Zych, Dept. of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, United States. Additional authors for this research include A. Domling and V. Ayyavoo.
Keywords for this news article include: HIV/AIDS, Pittsburgh, RNA Viruses, Pennsylvania, Retroviridae, United States, HIV Infections, Viral Proteins, Vertebrate Viruses, Primate Lentiviruses, North and Central America, Human Immunodeficiency Virus, Viral Sexually Transmitted Diseases, Immune System Diseases and Conditions.
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