By a News Reporter-Staff News Editor at Biotech Business Week -- According to news reporting originating from Alexandria, Virginia, by NewsRx journalists, a patent by the inventors Harder, Chris (Ottawa, CA); Cloake, Martin (Ottawa, CA); Perreault, Michel (Ottawa, CA); Lem, Paul (Ottawa, CA); Shayanpour, Alan (Stittsville, CA), filed on April 4, 2010, was published online on September 10, 2013 (see also Spartan Bioscience Inc.).
The assignee for this patent, patent number 8528777, is Spartan Bioscience Inc. (Ottawa, Ontario, CA).
Reporters obtained the following quote from the background information supplied by the inventors: "This invention relates to reaction tubes which are used in performing the Polymerase Chain Reaction (PCR) in thermal cyclers utilizing temperature controlled heat blocks for heating and cooling.
"Thin-walled 0.2 ml polypropylene reaction tubes are the standard for performing the Polymerase Chain Reaction (PCR) in thermal cyclers utilizing temperature-controlled heat blocks for heating and cooling, as disclosed, for example, in U.S. Pat. No. 3,725,010 patented Apr. 3, 1973 by Beckman Instruments Inc. Typically, the reaction tube is inserted into a well in the heat block so that the sides are surrounded by the heat block but the cap is not in contact with the heat block. Since the cap is not in contact with the heat block, it is cooler than the sides of the tube. There are a number of solutions that have been invented to minimize evaporation and condensation of liquid reagents into the cap during the heating and cooling cycles of PCR.
"These patented solutions include:
"U.S. Pat. No. 7,081,600, patented Jul. 25, 2006 by Stratagene California for 'Method and Apparatus for Cover Assembly for Thermal Cycling of Samples'. That patent provided a cover assembly for heating a plurality of samples which included a plurality of heater element areas located within a housing, and a force distribution system that engaged the plurality of heater element areas and distributed a force over the plurality of heater element areas, so that the cover assembly flexibly engaged the plurality of samples and heated the plurality of samples. The arrangement of the resistive heater, the heater backing plate, the force distribution system and the support plate allegedly provided substantial temperature uniformity among a plurality of sample tubes for receiving samples of biological material. The flexible heating cover assembly was alleged to improve the uniformity, efficiency, quality, reliability and controllability of the thermal response during thermal cycling of the biological material.
"U.S. Pat. No. 6,878,905, patented Apr. 12, 2005 by Stratagene California for 'Apparatus and Method For Flexible Heating Cover Assembly For Thermal Cycling of Samples of Biological Material'. The patent provided a flexible heating cover assembly for an apparatus for thermal cycling of samples of biological material which included: a housing including a plurality of assembly skirt components; a resistive heater located within the housing, the resistive heater including at least one outer heater element area and at least one central heater element area; a heater backing plate connected to the resistive heater to protect the resistive heater; a load sharing system engaging the heater backing plate to promote uniform contact of the resistive heater with a plurality of sample tubes; and a support plate aligning the assembly skirt components, so that the flexible heating cover assembly provides non-uniform heat distribution among the samples of biological material. The flexible heating cover assembly was alleged to improve the uniformity, efficiency, quality, reliability and controllability of the thermal response during thermal cycling of the biological material.