By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Enzymes and Coenzymes. According to news originating from Ishikawa, Japan, by NewsRx correspondents, research stated, "Flavonoids possess diverse health-promoting benefits but are nearly absent from rice, because most of the genes encoding enzymes for flavonoid biosynthesis are not expressed in rice seeds. In the present study, a transgenic rice plant producing several classes of flavonoids in seeds was developed by introducing multiple genes encoding enzymes involved in flavonoid synthesis, from phenylalanine to the target flavonoids, into rice."
Our news journalists obtained a quote from the research from Hokuriku University, "Rice accumulating naringenin was developed by introducing phenylalanine ammonia lyase (PAL) and chalcone synthase (CHS) genes. Rice producing other classes of flavonoids, kaempferol, genistein, and apigenin, was developed by introducing, together with PAL and CHS, genes encoding flavonol synthase/flavanone-3-hydroxylase, isoflavone synthase, and flavone synthases, respectively. The endosperm-specific GluB-1 promoter or embryo- and aleurone-specific 18-kDa oleosin promoters were used to express these biosynthetic genes in seed. The target flavonoids of naringenin, kaempferol, genistein, and apigenin were highly accumulated in each transgenic rice, respectively. Furthermore, tricin was accumulated by introducing hydroxylase and methyltransferase, demonstrating that modification to flavonoid backbones can be also well manipulated in rice seeds. The flavonoids accumulated as both aglycones and several types of glycosides, and flavonoids in the endosperm were deposited into PB-II-type protein bodies."
According to the news editors, the research concluded: "Therefore, these rice seeds provide an ideal platform for the production of particular flavonoids due to efficient glycosylation, the presence of appropriate organelles for flavonoid accumulation, and the small effect of endogenous enzymes on the production of flavonoids by exogenous enzymes."
For more information on this research see: Transgenic rice seed synthesizing diverse flavonoids at high levels: a new platform for flavonoid production with associated health benefits. Plant Biotechnology Journal, 2013;11(6):734-746. Plant Biotechnology Journal can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Plant Biotechnology Journal - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1467-7652)
The news correspondents report that additional information may be obtained from Y. Ogo, Hokuriku Univ, Dept. of Microbiol & Immunol, Kanazawa, Ishikawa, Japan. Additional authors for this research include K. Ozawa, T. Ishimaru, T. Murayama and F. Takaiwa (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Japan, Ishikawa, Synthase, Enzymes and Coenzymes
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