By a News Reporter-Staff News Editor at Pain & Central Nervous System Week -- A new study on Central Nervous System Diseases is now available. According to news reporting originating in Shandong, People's Republic of China, by NewsRx journalists, research stated, "Transmission of human cytomegalovirus (HCMV) to the fetus is the most common type of intrauterine infection; the mechanism of HCMV pathogenesis in the developing central nervous system remains unclear. Thrombospondins 1 and 2 (TSP1, TSP2) produced by immature astrocytes are critical for fetal synaptogenesis."
The news reporters obtained a quote from the research from the Qingdao University College of Medicine, "To examine the effect of HCMV on fetal astrocytes, human fetal astrocytes were isolated and cultured with HCMV AD169. Cells were harvested at different time points. Protein and mRNA expressions of TSP1 and TSP2 were determined using RT-qPCR, western blotting analysis, and enzyme-linked immunosorbent assay. The results showed that HCMV infection induced time-dependent decreases in mRNA and protein expressions of both TSP1 and TSP2 in astrocytes. Flow cytometry was used to detect apoptosis of HCMV-infected astrocytes, and the result indicated that there was no linkage between cell apoptosis and the decrease in TSP1 and TSP2 expressions induced by HCMV infection. When ganciclovir treatment was performed on HCMV-infected astrocytes, results showed that ganciclovir treatment inhibited the reduction of TSP1 and TSP2 expression in astrocytes."
According to the news reporters, the research concluded: "In the further study, pEGFP-N3-IE1 was transfected into astrocytes to identify that it was not IE1 but active viral replication that was essential in the continuous decrease of TSP1 and TSP2 expressions in HCMV-infected astrocytes."
For more information on this research see: Human cytomegalovirus infection modulates thrombospondins 1 and 2 in primary fetal astrocytes. Neuroreport, 2013;24(10):526-35. (Lippincott Williams and Wilkins - www.lww.com; Neuroreport - journals.lww.com/neuroreport/pages/default.aspx)
Our news correspondents report that additional information may be obtained by contacting L. Zhang, Dept. of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao, Shandong, People's Taiwan. Additional authors for this research include L. Li, B. Wang, D.M. Qian, X.M. Song and M. Hu (see also Central Nervous System Diseases).
Keywords for this news article include: Asia, Viral, Shandong, Virology, Neuroglia, Treatment, Astrocytes, DNA Viruses, Betaherpesvirinae, DNA Virus Infections, Human Cytomegalovirus, Herpesviridae Infections, People's Republic of China, Cytomegalovirus Infections, Central Nervous System Diseases, Central Nervous System Infections.
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