By a News Reporter-Staff News Editor at Science Letter -- Data detailed on Enzymes and Coenzymes have been presented. According to news reporting originating in Guilin, People's Republic of China, by NewsRx journalists, research stated, "A CE method based on a dual-enzyme co-immobilized capillary microreactor was developed for the simultaneous screening of multiple enzyme inhibitors. The capillary microreactor was prepared by co-immobilizing adenosine deaminase and xanthine oxidase on the inner wall at the inlet end of the separation capillary."
The news reporters obtained a quote from the research from Guangxi Normal University, "The enzymes were first immobilized on gold nanoparticles, and the functionalized gold nanoparticles were then assembled on the inner wall at the inlet end of the separation capillary treated with polyethyleneimine. With the developed CE method, the substrates and products were baseline separated within 3 min. The activity of the immobilized enzyme can be directly detected by measuring the peak height of the products. A statistical parameter Z' factor was recommended for evaluation of the accuracy of a drug screening system. In the present study, it was calculated to be larger than 0.5, implying a good accuracy. Finally, screening a small compound library containing two known enzyme inhibitors and 20 natural extracts by the proposed method was demonstrated."
According to the news reporters, the research concluded: "The known inhibitors were identified, and some natural extracts were found to be positive for two-enzyme inhibition by the present method."
For more information on this research see: Preparation of a dual-enzyme co-immobilized capillary microreactor and simultaneous screening of multiple enzyme inhibitors by capillary electrophoresis. Journal of Separation Science, 2013;36(15):2538-2543. Journal of Separation Science can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell - www.wiley.com/; Journal of Separation Science - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1615-9314)
Our news correspondents report that additional information may be obtained by contacting P.T. Lin, Guangxi Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Chem & Mol Engn Med Resources, Guilin 541004, People's Republic of China. Additional authors for this research include S.L. Zhao, X. Lu, F.G. Ye and H.S. Wang (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Guilin, Nanoparticle, Nanotechnology, Emerging Technologies, Enzymes and Coenzymes, Capillary Electrophoresis, People's Republic of China
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