By a News Reporter-Staff News Editor at Biotech Week -- New research on Oncology is the subject of a report. According to news reporting originating from Nashville, Tennessee, by NewsRx correspondents, research stated, "Apoptosis is irreversible programmed cell death, characterized by a cellular cascade activation of caspase 3, which subsequently degrades proteins and other components of cells with a motif sequence. Here we report a novel reporter system to detect apoptosis, growth arrest, and cell death based on controlled and self-amplified protein degradation."
Our news editors obtained a quote from the research from Vanderbilt University, "The key element of the reporter system is an apoptotic sensor chimerical protein which consists of three components: procaspase 3, ubiquitin (Ub), and a strong consensus sequence of N-degron. Between each of these units is a DEVD (Asp-Glu-Val-Asp) sequence, which acts as the cleavage target of caspase 3. This non-conventional signal loss approach is much more sensitive than other native methods that are based on signal gain. The superior sensitivity is demonstrated by its effective application in 386-well high-throughput screening (HTS) with low drug concentrations and a short incubation time. The HTS selection process using this reporter system is very simple and economic. The simplicity eliminates potential errors introduced by multiple steps; there is no need for any substrate. Furthermore, the cells in the assay need not be disrupted, and the morphology of the cells can provide additional information on mechanisms. After HTS, the intact cells can also be used for other analytic analysis. This system thus has a potentially important role in the discovery and development of new anticancer drugs."
According to the news editors, the research concluded: "It also appears to be very versatile, can be used both in vitro and in vivo with different linked reporter genes, and can be used for a variety of imaging applications."
For more information on this research see: A Novel Reporter System for Molecular Imaging and High-Throughput Screening of Anticancer Drugs. Chembiochem, 2013;14(12):1494-1503. Chembiochem can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell - www.wiley.com/; Chembiochem - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1439-7633)
The news editors report that additional information may be obtained by contacting J.P. Xie, Vanderbilt University, Dept. of Mol Physiol & Biophys, Dept. of Phys & Astron, Dept. of Biomed Engn, Nashville, TN 37232, United States. Additional authors for this research include C.X. Wang, J. Virostko, H.C. Manning, W. Pham, J. Bauer and J.C. Gore (see also Oncology).
Keywords for this news article include: Therapy, Oncology, Nashville, Tennessee, United States, Nanotechnology, Molecular Imaging, Emerging Technologies, North and Central America
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