By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Nanoparticles have been published. According to news originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "Recently, the applications of nanomaterial-assisted polymerase chain reaction (nanoPCR) have received considerable attention. Several potential mechanisms have been proposed, but mainly according to the results of PCR assays under specific conditions and lacking direct and general evidence."
Our news journalists obtained a quote from the research from Capital Normal University, "The mechanism of nanoPCR has not been elucidated yet. Here, taking gold nanoparticles (AuNPs) as an example, we report the three general effects of AuNPs: (1) AuNPs adsorb polymerase and modulate the amount of active polymerase in PCR, which was directly demonstrated by a simple and straightforward colorimetric assay and the dynamic light scattering measurements. (2) AuNPs adsorb primers and decrease the melting temperatures (T-m) of the duplexes formed with perfectly matched and mismatched primers and increase the T-m difference between them. (3) AuNPs adsorb PCR products and facilitate the dissociation of them in the denaturing step. All these effects were confirmed by addition of a rationally selected surface adsorbent, bovine thrombin, to highly efficiently modulate the surface adsorption of PCR components. These findings suggested that AuNPs should have multiple effects on PCR: (1) to regulate PCR in a case-by-case way via modulating the amount of active polymerase in PCR; (2) to improve PCR specificity in the annealing step via increasing the T-m, difference between the perfectly matched and mismatched primers; (3) to improve PCR efficiency via speeding up the dissociation of the PCR products in the denaturing step. Taken together, we proposed the mechanism of nanoPCR is that the surface interaction of PCR components (polymerase, primers, and products) with AuNPs regulates nanoPCR We further demonstrated that the applications of these findings improve the PCR of the amelogenin genes and Hepatitis B virus gene for genetic analysis."
According to the news editors, the research concluded: "These findings could also provide helpful insight for the applications of other nanomaterials in nanoPCR."
For more information on this research see: Mechanism Studies on NanoPCR and Applications of Gold Nanoparticles in Genetic Analysis. ACS Applied Materials & Interfaces, 2013;5(13):6276-6284. ACS Applied Materials & Interfaces can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Applied Materials & Interfaces - www.pubs.acs.org/journal/aamick)
The news correspondents report that additional information may be obtained from X.H. Lou, Capital Normal Univ, Dept. of Chem, Beijing 100048, People's Republic of China (see also Nanoparticles).
Keywords for this news article include: Asia, Beijing, Genetics, Polymerase, Nanotechnology, Gold Nanoparticles, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China
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