By a News Reporter-Staff News Editor at Health & Medicine Week -- Research findings on Cytometry are discussed in a new report. According to news originating from Leeds, United Kingdom, by NewsRx correspondents, research stated, "Assessing dose in nanoparticle-cell interactions is inherently difficult due to a complex multiplicity of possible mechanisms and metrics controlling particle uptake. The fundamental unit of nanoparticle dose is the number of particles internalized per cell; we show that this can be obtained for large cell populations that internalize fluorescent nanoparticles by endocytosis, through calibration of cytometry measurements to transmission electron microscopy data."
Our news journalists obtained a quote from the research from the University of Leeds, "Low-throughput, high-resolution electron imaging of quantum dots in U-2 OS cells is quantified and correlated with high-throughput, low-resolution optical imaging of the nanoparticle-loaded cells. From the correlated data, we obtain probability distribution functions of vesicles per cell and nanoparticles per vesicle. Sampling of these distributions and comparison to fluorescence intensity histograms from flow cytometry provide the calibration factor required to transform the cytometry metric to total particle dose per cell, the mean value of which is 2.4 million."
According to the news editors, the research concluded: "Use of the probability distribution functions to analyze particle partitioning during cell division indicates that, while vesicle inheritance is near symmetric highly variable vesicle loading leads to a highly asymmetric particle dose within the daughter cells."
For more information on this research see: Quantification of Nanoparticle Dose and Vesicular Inheritance in Proliferating Cells. ACS Nano, 2013;7(7):6129-6137. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Nano - www.pubs.acs.org/journal/ancac3)
The news correspondents report that additional information may be obtained from H.D. Summers, University of Leeds, Sch Proc Environm & Mat Engn, Inst Mat Res, Leeds LS2 9JT, W Yorkshire, United Kingdom. Additional authors for this research include M.R. Brown, M.D. Holton, J.A. Tonkin, N. Hondow, A.P. Brown, R. Brydson and P. Rees (see also Cytometry).
Keywords for this news article include: Leeds, Europe, Cytometry, Nanoparticle, United Kingdom, Nanotechnology, Emerging Technologies
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