By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Researchers detail new data in Stem Cell Research. According to news reporting out of Tehran, Iran, by NewsRx editors, research stated, "The objective of this study was to evaluate the potential of this type of recombinant lentivirus to generate glucose-responsive insulin producing cells in vitro. All steps of cloning were confirmed using restriction digests."
Our news journalists obtained a quote from the research from Pasteur Institute, "After the transduction, mesenchymal stem cells gradually began to change their morphology and showed differentiation into islet like structures. RT-PCR results confirmed the expression of insulin1, insulin2 and pdx-1 in differentiated cells. Dithizone staining of mouse MSCs showed the concentration of glucose in islet like structures. ELISA analysis validated the insulin secretion of islet like structures which in the high-glucose medium (25 mmol/l) was 7.44 fold higher than that secreted in the low-glucose medium (5 mmol/l). Our results demonstrated that mouse mesenchymal stem cells can be differentiated into effective glucose-responsive insulin producing cells through our new recombinant lentiviral transduction of pdx-1 gene in vitro."
According to the news editors, the research concluded: "This new lentiviral vector could be suggested as an effective candidate for using in gene therapy of type-1 diabetes."
For more information on this research see: In vitro generation of glucose-responsive insulin producing cells using lentiviral based pdx-1 gene transduction of mouse (C57BL/6) mesenchymal stem cells. Biochemical and Biophysical Research Communications, 2013;437(3):413-419. Biochemical and Biophysical Research Communications can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Biochemical and Biophysical Research Communications - www.elsevier.com/wps/product/cws_home/622790)
Our news journalists report that additional information may be obtained by contacting S. Rahmati, Pasteur Inst Iran, Dept. of Biochem, Tehran 13164, Iran. Additional authors for this research include N. Alijani and M. Kadivar (see also Stem Cell Research).
Keywords for this news article include: Iran, Asia, Biotechnology, Tehran, Proinsulin, Gene Therapy, Bioengineering, Peptide Hormones, Stem Cell Research, Mesenchymal Stem Cells
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