By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Enzymes and Coenzymes is the subject of a report. According to news reporting out of Mexico City, Mexico, by NewsRx editors, research stated, "There is growing interest in developing high-yield and low-cost production of xylanolytic enzymes for industrial applications using agroindustrial byproducts. A native strain of Aspergillus niger GS1 was used to produce beta-xylosidase (EC 188.8.131.52) on solid state fermentation using corn pericarp (CP) with innovative alkaline electrolyzed water (AEW) pretreatment at room temperature. beta-xylosidase was purified by ammonium sulfate fractionation followed by anion exchange and hydrophobic interaction chromatographies. beta-Xylosidase showed a molecular weight of 111 kDa, isoelectric point of 5.35 and specific ctivity of 386.7 U (mg protein)(-1), using beta-nitrophenyl-beta-b-xylopyranoside as substrate, at pH 5 and 60 degrees C, and optimal activity at pH 4.5."
Our news journalists obtained a quote from the research from Metropolitan Autonomous University, "Optimal temperature was 65 C, showing full activity after 1 h at 60 degrees C. Activity was reduced by 1 mM beta-mercaptoethanol (55.6 0.1%), and enhanced by 1 mM SDS (11.0 +/- 0.03%). K-m and V-max were 6.1 0.9 mM and 1364 +/- 105 U (mg protein)(-1), respectively, whereas kat was 5.1 s(-1)."
According to the news editors, the research concluded: "A predominant alpha-helix (41%) was determined from circular dichroism on beta-xylosidase, while thermal transition profiles produced a T-m of 54.1 +/- 5.8 degrees C, enthalpy change for unfolding of 67.4 +/- 6.7 kJ/mol, and onset temperature of 37 degrees C. Pre-treatment of CP using AEW is an ecologically friendly alternative to chemical and heat treatments for the production of relatively high levels of beta-xylosidase."
For more information on this research see: Isolation and properties of beta-xylosidase from Aspergillus niger GS1 using corn pericarp upon solid state fermentation. Process Biochemistry, 2013;48(7):1018-1024. Process Biochemistry can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Process Biochemistry - www.elsevier.com/wps/product/cws_home/422857)
Our news journalists report that additional information may be obtained by contacting F.I. Diaz-Malvaez, Univ Autonoma Metropolitana Iztapalapa, Dept. of Quim, Mexico City 09340, DF, Mexico. Additional authors for this research include B.E. Garcia-Almendarez, A. Hernandez-Arana, A. Amaro-Reyes and C. Regalado-Gonzalez (see also Enzymes and Coenzymes).
Keywords for this news article include: Mexico City, Xylosidases, Glycoside Hydrolases, Enzymes and Coenzymes, North and Central America
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