By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Microbial Technology have been published. According to news reporting originating in Inchon, South Korea, by NewsRx journalists, research stated, "For the commercialization of plant-made pharmaceuticals (PMPs) using transgenic plant cell cultures, the establishment of a cell-banking system has been known to be an essential process. Plant cells are traditionally maintained by repeated subcultures."
The news reporters obtained a quote from the research from Inha University, "However, this method has several problems including genetic instability of transformed cell lines, time- and cost-consuming. In this study, long-term cryopreserved rice suspension cells were firstly investigated for the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig). The cryopreserved cells for 5 years were regrowed to callus successfully and then suspended into the liquid medium. Consequently, the maximum cell mass and the hCTLA4Ig production were similar levels compared to those of the non-cryopreserved cells (control) even though hCTLA4Ig productivity was 1.7-fold higher than that of control. To further assess the level of improvements in hCTLA4Ig productivity in cryopreserved cells, hCTLA4Ig production profiles were statistically assessed between data of the cryopreserved cells for 5 years and annual data of non-cryopreserved cells maintained by subculture for 5 years. These results also indicate that hCTLA4Ig productivity in cryopreserved cells for 5 years was significantly increased (p-value:
According to the news reporters, the research concluded: "We show that the long-term cryopreservation of transgenic rice cells was possible to support stable cell lines for the production of PMPs."
For more information on this research see: Assessment of long-term cryopreservation for production of hCTLA4Ig in transgenic rice cell suspension cultures. Enzyme and Microbial Technology, 2013;53(3):216-222. Enzyme and Microbial Technology can be contacted at: Elsevier Science Inc, 360 Park Ave South, New York, NY 10010-1710, USA. (Elsevier - www.elsevier.com; Enzyme and Microbial Technology - www.elsevier.com/wps/product/cws_home/525004)
Our news correspondents report that additional information may be obtained by contacting J.Y. Kwon, Inha University, Dept. of Biol Engn, Inchon 402751, South Korea. Additional authors for this research include S.H. Jeong, J.W. Choi, Y.Y. Pak and D.I. Kim (see also Microbial Technology).
Keywords for this news article include: Asia, Inchon, South Korea, Microbial Technology
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