By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Immunology have been published. According to news reporting from Shenyang, People's Republic of China, by NewsRx journalists, research stated, "Human Toll-like receptor 2 (TLR2) is a receptor for a variety of microbial products and mediates activation signals in cells of the innate immune system. Therefore, it is of great interest to investigate the molecular mechanisms that control the expression of TLR2."
The news correspondents obtained a quote from the research from China Medical University and Hospital, "In this study, using real-time PCR and western blot assays, we show that trichostatin A (TSA), which is a histone deacetylase inhibitor, upregulates the expression of both TLR2 mRNA and protein in the human THP-1 cell line. A luciferase activity analysis of the truncated TLR2 promoter indicated that the region from -230 to -140 in the TLR2 promoter was sensitive to TSA. Moreover, using electrophoresis mobility shift and chromatin immunoprecipitation assays, we identified an AP-2 alpha (AP-2 alpha) responsive element at position -184 and found that the binding of AP-2 alpha to this element was enhanced by TSA under in vitro and in vivo conditions. Immunoprecipitation and western blot analyses showed that the levels of acetylated AP-2 alpha were increased in THP-1 cells after TSA treatment, and this increase is consistent with the increased binding affinity to the AP-2 alpha responsive elements. In summary, these data define a mechanism through which AP-2 alpha acetylation and increased promoter access induce the expression of the TLR2 gene."
According to the news reporters, the research concluded: "This mechanism may provide insight into a regulatory mode of TLR2 expression and the molecular foundations of certain immunological diseases."
For more information on this research see: Upregulation of Toll-like receptor 2 gene expression by acetylation of AP-2 alpha in THP-1 cells, a human monocytic cell line. International Journal of Biochemistry & Cell Biology, 2013;45(8):1594-1599. International Journal of Biochemistry & Cell Biology can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. (Elsevier - www.elsevier.com; International Journal of Biochemistry & Cell Biology - www.elsevier.com/wps/product/cws_home/395)
Our news journalists report that additional information may be obtained by contacting M. Li, China Med Univ, Coll Basic Med Sci, Shenyang 110001, People's Republic of China. Additional authors for this research include X. Li, E.H. Wang and E.J. Luo (see also Immunology).
Keywords for this news article include: Asia, Shenyang, Immunology, Membrane Proteins, Toll-Like Receptor 2, People's Republic of China, Pattern Recognition Receptors
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