By a News Reporter-Staff News Editor at Biotech Week -- A new study on Biotechnology is now available. According to news reporting originating in Gujarat, India, by NewsRx journalists, research stated, "An isotope dilution high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous determination of oxybutynin and its pharmacologically active metabolite N-desethyl oxybutynin in human plasma. Extraction of oxybutynin, its metabolite and their deuterated analogs as internal standards (ISs) from 300 mu L human plasma was carried out by liquid-liquid extraction with methyl tert-butyl ether-ethyl acetate solvent mixture."
The news reporters obtained a quote from the research from St. Xavier's College, "Chromatographic separation of analytes was performed on Cosmosil C18 (150 mm x 4.6 mm, 5 mu m) column under isocratic conditions with acetonitrile-1.0 mM ammonium acetate (90:10, v/v) as the mobile phase. Six endogenous plasma phospholipids (4963/184.0, 524.3/184.0, 758.5/184.0, 786.5/184.0,806.5/184.0 and 810.5/184.0) were monitored to determine the extraction efficiency under different extraction conditions. The precursor ? product ion transition for both the analytes and ISs were monitored on a triple quadrupole mass spectrometer, operating in the multiple reaction monitoring and positive ionization mode. The method was validated over a concentration range of 0.050-10.0 ng/mL for oxybutynin and 0.500-100 ng/mL for N-desethyl oxybutynin. The mean extraction recovery for analytes (80.4%) and ISs (76.9%) was consistent across five QC levels. Bench top, wet and dry extract, freeze-thaw and long term stability was evaluated for both the analytes. The method was applied to support a bioequivalence study of 5 mg tablet formulation in 74 healthy Indian subjects."
According to the news reporters, the research concluded: "Assay reproducibility was demonstrated by reanalysis of 344 incurred samples."
For more information on this research see: Simultaneous analysis of oxybutynin and its active metabolite N-desethyl oxybutynin in human plasma by stable isotope dilution LC-MS/MS to support a bioequivalence study. Journal of Pharmaceutical and Biomedical Analysis, 2013;84():244-255. Journal of Pharmaceutical and Biomedical Analysis can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Pharmaceutical and Biomedical Analysis - www.elsevier.com/wps/product/cws_home/525434)
Our news correspondents report that additional information may be obtained by contacting P. Sharma, St Xaviers College, Dept. of Chem, Ahmadabad 380009, Gujarat, India. Additional authors for this research include D.P. Patel, M. Sanyal, H. Berawala, S. Guttikar and P.S. Shrivastav (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, India, Gujarat
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