By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Epithelial Cells is now available. According to news reporting originating in Yangling, People's Republic of China, by NewsRx journalists, research stated, "Transgenic animals that express antimicrobial agents in their milk can inhibit bacterial pathogens that cause mastitis. Our objective was to produce human ?-defensin-3 (HBD3) transgenic embryos by nuclear transfer using goat mammary epithelial cells (GMECs) as donor cells."
The news reporters obtained a quote from the research from Northwest A&F University, "Three GMEC lines (GMEC1, GMEC2, and GMEC3) were transfected with a HBD3 mammary-specific expression vector by electroporation. There was a difference (p 6 cells, respectively). After inducing expression, the mRNA and protein of HBD3 were detected by reverse transcription polymerase chain reaction and Western blot analysis in transgenic cells. Transgenic clonal cells expressing HBD3 were used as donor cells to investigate development of cloned embryos. There were no significant differences in rates of cleavage or blastocyst formation of cloned embryos from transgenic (GMEC1T2 and GMEC2T3) and nontransgenic (GMEC1 and GMEC2) GMECs (72.3 ± 5.0%, 69.5 ± 2.3%, 61.8 ± 4.8%, and 70.0 ± 2%; and 16.8 ± 0.5%, 17.5 ± 0.7%, 16.7 ± 0.9%, and 17.5 ± 0.6%, respectively). However, the fusion rate, cleavage rate, and blastocyst formation rate of cloned embryos from a transgenic clonal cell line (GMEC2T6, 50.7 ± 2.1%, 55.5 ± 2.0%, and 11.1 ± 0.6%) were lower than those of other groups (p
According to the news reporters, the research concluded: "However, transgenic GMECs expressing HBD3 might be used as donor cells for producing transgenic goats that express increased concentrations of ?-defensins in their milk."
For more information on this research see: Production of cloned embryos from caprine mammary epithelial cells expressing recombinant human ?-defensin-3. Theriogenology, 2013;79(4):660-6. (Elsevier - www.elsevier.com; Theriogenology - www.elsevier.com/wps/product/cws_home/525024)
Our news correspondents report that additional information may be obtained by contacting J. Liu, College of Veterinary Medicine, Northwest A&F University, Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling, Shaanxi, People's Taiwan. Additional authors for this research include Y. Luo, Q. Liu, L. Zheng, Z. Yang, Y. Wang, J. Su, F. Quan and Y. Zhang (see also Epithelial Cells).
Keywords for this news article include: Asia, Yangling, Epithelial Cells, People's Republic of China.
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