By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Neutral Amino Acids have been published. According to news reporting originating in Himachal Prades, India, by NewsRx journalists, research stated, "The first step in Agrobacterium tumefaciens infection of plants is the establishment of cell-cell contact between the two partners. However, failure to establish such contact makes many plants and explants recalcitrant to A. tumefaciens infection."
The news reporters obtained a quote from the research from the Institute of Himalayan Bioresource Technology, "Tea is one such example where even the popular inducer, acetosyringone failed to facilitate A. tumefaciens infection due to the presence of high amounts of bactericidal/bacteriostatic polyphenols. Quinones are formed as a result of polyphenols oxidation. They cause tissue browning and necrosis during the process of transformation, and in turn prevent A. tumefaciens infection. Compounds such as polyphenol adsorbents, i.e., polyvinylpyrrolidone and charcoal, and antioxidants like cysteine and ascorbic acid were screened to overcome tissue browning. Although these compounds enhanced the growth of A. tumefaciens, these failed to facilitate the infection of the leaves of either Kangra Jat, Tocklai Variety-1, UPASI-9, UPASI-10, and Stock-449 cultivars of tea. On the contrary, the presence of filter sterilized l-glutamine and l-glutamic acid in the co-cultivation medium facilitated successful A. tumefaciens infection of recalcitrant tea leaves. l-Glutamine and glutamic acid form harmless adducts by binding to quinones."
According to the news reporters, the research concluded: "Therefore, their presence in the co-cultivation medium allowed the tea leaves to remain living and appealing to the infecting A. tumefaciens. Successful A. tumefaciens infection of tea leaves was confirmed by positive signals in GUS assay, PCR, and Dot blot."
For more information on this research see: L-Glutamine and L-Glutamic Acid Facilitate Successful Agrobacterium Infection of Recalcitrant Tea Cultivars. Applied Biochemistry and Biotechnology, 2013;170(7):1649-1664. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)
Our news correspondents report that additional information may be obtained by contacting N. Kumar, CSIR, Inst Himalayan Bioresource Technol, Div Biotechnol, Palampur 176061, Himachal Prades, India. Additional authors for this research include A. Gulati and A. Bhattacharya (see also Neutral Amino Acids).
Keywords for this news article include: Asia, India, Glutamine, Himachal Prades, Basic Amino Acids, Diamino Amino Acids, Neutral Amino Acids
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