By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Proteins is the subject of a report. According to news reporting out of Shijiazhuang, People's Republic of China, by NewsRx editors, research stated, "Cross-linked enzyme aggregates (CLEAs) have been recently proposed as an alternative to conventional immobilization methods on solid carriers. However, the low cross-linking efficiency causes the major activity loss and instability in the conventional protocol for CLEA preparation."
Our news journalists obtained a quote from the research from Hebei University, "Herein, the effects of bovine serum albumin and starch addition on the cross-linking efficiency of CLEAs of phenylalanine ammonia lyase (PAL) from Rhodotorula glutinis were evaluated. A co-aggregation strategy was developed to improve cross-linking efficiency by adding starch and bovine serum albumin (BSA). CLEAs of PAL prepared in the presence of BSA and starch (PSB-CLEAs) retained 36 % activity, whereas CLEAs prepared without BSA and starch (PAL-CLEAs) retained only 8 % activity of the starting enzyme preparation. Compared with PAL-CLEAs, the thermal stability of PSB-CLEAs has improved considerably, maintaining 30 % residual activity after 4 h of incubation at 70 A degrees C, whereas the PAL-CLEAs have only 13 % residual activity. PSB-CLEAs also exhibited the expected increased stability of PAL against hydrophilic organic solvents, superior operability, and higher storage stability."
According to the news editors, the research concluded: "The proposed technique of preparing CLEAs using co-aggregation with starch and BSA would rank among the potential strategies for efficiently preparing robust and highly stable enzyme aggregates."
For more information on this research see: A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin. Applied Biochemistry and Biotechnology, 2013;170(8):1827-1837. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)
Our news journalists report that additional information may be obtained by contacting J.D. Cui, Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, People's Republic of China. Additional authors for this research include L.M. Sun and L.L. Li (see also Proteins).
Keywords for this news article include: Asia, Shijiazhuang, Ammonia Lyases, Blood Proteins, Nitrogen Compounds, Acute-Phase Proteins, Aromatic Amino Acids, Bovine Serum Albumin, Enzymes and Coenzymes, Essential Amino Acids, Carbon-Nitrogen Lyases, People's Republic of China, Phenylalanine Ammonia-Lyase
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