By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biotechnology. According to news reporting from Warsaw, Poland, by NewsRx journalists, research stated, "Biofilm formation is a well-known problem in management of metalworking fluid systems. Due to persistence of microorganisms within biofilms, the reappearance of various species of bacteria, including nontuberculous mycobacteria is often observed after the use of biocides and/or cleaning of delivery systems and replacement of cooling fluid."
The news correspondents obtained a quote from the research from the Department of Microbiology, "The aim of this study was to determine the usefulness of the tetrazolium salt assay (MTT assay) for assessing the viability of bacteria in biofilms formed in vitro in fresh and used cutting oils, as well as their susceptibility to antimicrobial biocides. Biofilms were established in the microtiter plate format. The results showed that quantification of formazan, a product of the tetrazolium salt reduction by electron transport system could be used for determination of the propensity of bacteria to form biofilms in these complex media. The use of the assay allows also determination of antimicrobial activity of biocides against biofilms in fresh and used metalworking fluids. Biofilms produced by Gram-negative isolates recovered from field metalworking fluids as well as the wild bacterial communities differed in metabolic activity depending on the type of fresh coolants. The MTT assay has high-throughput potential and can be efficiently used for determination of biofilm-forming capacity of microorganisms from individual machines in metalworking industry."
According to the news reporters, the research concluded: "The use of the assay may also guide the selection of the most appropriate biocide to fight these microorganisms."
For more information on this research see: Use of MTT assay for determination of the biofilm formation capacity of microorganisms in metalworking fluids. World Journal of Microbiology & Biotechnology, 2013;29(9):1635-1643. World Journal of Microbiology & Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA (see also technology.html">Biotechnology).
Our news journalists report that additional information may be obtained by contacting E.A. Trafny, Military Inst Hyg & Epidemiol, Dept. of Microbiol, PL-01163 Warsaw, Poland. Additional authors for this research include R. Lewandowski, I. Zawistowska-Marciniak and M. Stepinska.
Keywords for this news article include: Biotechnology, Warsaw, Poland, Europe
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC