By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Biomedicine and Biomedical Engineering. According to news reporting out of Frankfurt, Germany, by NewsRx editors, research stated, "Tissue Engineering is an important method for generating cartilage tissue with isolated autologous cells and the support of biomaterials. In contrast to various gel-like biomaterials, human demineralized bone matrix (DBM) guarantees some biomechanical stability for an application in biomechanically loaded regions."
Our news journalists obtained a quote from the research from Johann Wolfgang Goethe-University, "The present study combined for the first time the method of seeding chondrocyte-macroaggregates in DBM for the purpose of cartilage tissue engineering. After isolating human nasal chondrocytes and creating a three-dimensional macroaggregate arrangement, the DBM was cultivated in vitro with the macroaggregates. The interaction of the cells within the DBM was analyzed with respect to cell differentiation and the inhibitory effects of chondrocyte proliferation. In contrast to chondrocyte-macroaggregates in the cell-DBM constructs, morphologically modified cells expressing type I collagen dominated. The redifferentiation of chondrocytes, characterized by the expression of type II collagen, was only found in low amounts in the cell-DBM constructs. Furthermore, caspase 3, a marker for apoptosis, was detected in the chondrocyte-DBM constructs. In another experimental setting, the vitality of chondrocytes as related to culture time and the amount of DBM was analyzed with the BrdU assay. Higher amounts of DBM tended to result in significantly higher proliferation rates of the cells within the first 48 h. After 96 h, the vitality decreased in a dose-dependent fashion."
According to the news editors, the research concluded: "This study provides the proof of concept of chondrocyte-macroaggregates with DBM as an interesting method for the tissue engineering of cartilage. The as-yet insufficient redifferentiation of the chondrocytes and the sporadic initiation of apoptosis will require further investigations."
For more information on this research see: Cartilage tissue engineering of nasal septal chondrocyte-macroaggregates in human demineralized bone matrix. Cell and Tissue Banking, 2013;14(2):255-66. Cell and Tissue Banking can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Cell and Tissue Banking - www.springerlink.com/content/1389-9333/)
Our news journalists report that additional information may be obtained by contacting J. Liese, Dept. of General and Visceral Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590, Frankfurt, Germany. Additional authors for this research include U. Marzahn, K. El Sayed, A. Pruss, A. Haisch and K. Stoelzel (see also Biomedicine and Biomedical Engineering).
Publisher contact information for the journal Cell and Tissue Banking is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Tissue Engineering, Biomedicine and Biomedical Engineering, Europe, Germany, Frankfurt, Chondrocytes, Bone Research, Bioengineering, Connective Tissue Cells.
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