By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Enzymes and Coenzymes. According to news reporting from Groton, Connecticut, by NewsRx journalists, research stated, "2-Arachidonoylglycerol (2-AG) is a component of the endocannabinoid receptor pathway and is primarily hydrolyzed by monoacylglycerol lipase (MAGL) in vivo. We found that the non-specific serine esterase, butyrylcholinesterase (BChE), can hydrolyze 2-AG with reasonable affinity and may present a new compensatory mechanism for endocannabinoid regulation."
The news correspondents obtained a quote from the research from Groton Laboratories, "In vitro hydrolysis reactions of 2-AG with equine BChE were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) positive/negative electrospray ionization (ESI +/-) to measure the formation of arachidonic acid (AA) and the loss of 2-AG over time (min). The resulting Michaelis-Menten approximations reveal that BChE has affinity towards 2-AG in phosphate buffer at neutral pH (7.4). The calculated V-max, K-m and k(cat) were 12.1 nmol s(-1), 57.5 mu M, and 0.074 s(-1), respectively, which produced a diffusion-controlled rate of association (k(cat)/K-m) of 1.3 x 10(3) - M-1 s(-1). Human BChE 2-AG hydrolysis was measured by immunoprecipitating BChE from fresh plasma and monitoring 2-AG loss and AA formation over time."
According to the news reporters, the research concluded: "These findings show that BChE can hydrolyze 2-AG which may be evidence of a more specific role for BChE in endocannabinoid regulation."
For more information on this research see: 2-Arachidonoylglycerol is a substrate for butyrylcholinesterase: A potential mechanism for extracellular endocannabinoid regulation. Archives of Biochemistry and Biophysics, 2013;536(1):1-5. Archives of Biochemistry and Biophysics can be contacted at: Elsevier Science Inc, 360 Park Ave South, New York, NY 10010-1710, USA. (Elsevier - www.elsevier.com; Archives of Biochemistry and Biophysics - www.elsevier.com/wps/product/cws_home/622787)
Our news journalists report that additional information may be obtained by contacting J. Barricklow, Groton Labs, Pfizer Global Res & Dev, Groton, CT 06340, United States (see also Enzymes and Coenzymes).
Keywords for this news article include: Groton, Hydrolases, Connecticut, United States, Butyrylcholinesterase, Enzymes and Coenzymes, North and Central America
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC